In 1908, a massive explosion known as the Tunguska Event (TE) occurred in Central Siberia. However, its origin remains widely discussed and environmental impacts are not known in detail. We investigated evidence of the TE in sediments of Suzdalevo Lake, which is located near the explosion epicenter. According to local nomads (Evenkis), Suzdalevo Lake did not exist before the TE and was considered as a possible impact-origin water body. However, apart from oral testimony, there is no evidence of the lake formation process. Two short sediment cores (SUZ1 and SUZ3) were retrieved from the lake and dated using 210Pb and 137Cs. The sedimentary record was characterized using magnetic susceptibility, X-ray fluorescence, and the screening for melted magnetic microspherules. To study possible effects of the TE on the lake ecosystem, we performed diatom and freshwater fauna remains analyses. Results indicate that the lake contains sediments that originated before the TE and thus its formation was not related to the impact. Also, the depth to diameter ratio of the lake basin is too low (