دورية أكاديمية
Genome-wide identification, characterization and expression analysis of the non-specific lipid transfer proteins in potato
العنوان: | Genome-wide identification, characterization and expression analysis of the non-specific lipid transfer proteins in potato |
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المؤلفون: | Guojun Li, Menglu Hou, Yaxue Liu, Yue Pei, Minghui Ye, Yao Zhou, Chenxi Huang, Yaqi Zhao, Haoli Ma |
المصدر: | BMC Genomics, Vol 20, Iss 1, Pp 1-14 (2019) |
بيانات النشر: | BMC, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Biotechnology LCC:Genetics |
مصطلحات موضوعية: | Potato, nsLTP, Genome-wide, Tuber development, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
الوصف: | Abstract Background Plant non-specific lipid transfer proteins (nsLTPs) are small, basic proteins that are abundant in higher plants. They have been reported to play an important role in various plant physiological processes, such as lipid transfer, signal transduction, and pathogen defense. To date, a comprehensive analysis of the potato nsLTP gene family is still lacking after the completion of potato (Solanum tuberosum L.) genome sequencing. A genome-wide characterization, classification and expression analysis of the StnsLTP gene family was performed in this study. Results In this study, a total of 83 nsLTP genes were identified and categorized into eight types based on Boutrot’s method. Multiple characteristics of these genes, including phylogeny, gene structures, conserved motifs, protein domains, chromosome locations, and cis-elements in the promoter sequences, were analyzed. The chromosome distribution and the collinearity analyses suggested that the expansion of the StnsLTP gene family was greatly enhanced by the tandem duplications. Ka/Ks analysis showed that 47 pairs of duplicated genes tended to undergo purifying selection during evolution. Moreover, the expression of StnsLTP genes in various tissues was analyzed by using RNA-seq data and verified by quantitative real-time PCR, revealing that the StnsLTP genes were mainly expressed in younger tissues. These results indicated that StnsLTPs may played significant and functionally varied roles in the development of different tissues. Conclusion In this study, we comprehensively analyzed nsLTPs in potato, providing valuable information to better understand the functions of StnsLTPs in different tissues and pathways, especially in response to abiotic stress. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1471-2164 |
Relation: | http://link.springer.com/article/10.1186/s12864-019-5698-x; https://doaj.org/toc/1471-2164 |
DOI: | 10.1186/s12864-019-5698-x |
URL الوصول: | https://doaj.org/article/2eecadfbda03444594470cc284d59af1 |
رقم الأكسشن: | edsdoj.2eecadfbda03444594470cc284d59af1 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14712164 |
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DOI: | 10.1186/s12864-019-5698-x |