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Overexpression of the Bacillus licheniformis GroES enhances thermotolerance of Bacillus subtilis WB600

التفاصيل البيبلوغرافية
العنوان: Overexpression of the Bacillus licheniformis GroES enhances thermotolerance of Bacillus subtilis WB600
المؤلفون: Zixing Dong, Xiaoling Chen, Ke Cai, Peili Shen, Kangming Tian, Peng Jin, Xiaoguang Liu, Zhengxiang Wang
المصدر: Biotechnology & Biotechnological Equipment, Vol 32, Iss 6, Pp 1527-1532 (2018)
بيانات النشر: Taylor & Francis Group, 2018.
سنة النشر: 2018
المجموعة: LCC:Biotechnology
مصطلحات موضوعية: GroES, thermotolerance, Bacillus subtilis, heat shock protein, Biotechnology, TP248.13-248.65
الوصف: Use of thermotolerant strains offers the advantage of conducting production process at elevated temperatures, thereby improving the catalytic efficiency and reducing the energy consumption and the production costs. In the present study, thermotolerance gene groES from the thermophilic strain Bacillus licheniformis B186 was implanted into B. subtilis WB600 to improve its thermotolerance. The engineered strain WB600-pHY-groES displayed reduced doubling time as well as increased specific growth rates and cell viability compared with the control. Our results suggested that introducing heat shock proteins (HSPs) from thermophiles into B. subtilis could be an effective approach to enhance its thermotolerance, thus facilitating the development of multichaperone expression systems for constructing more thermotolerant commercial B. subtilis strains. To the best of our knowledge, this is the first report on improving the thermotolerance of B. subtilis by overexpression of HSPs.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1310-2818
1314-3530
13102818
76761835
Relation: https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530
DOI: 10.1080/13102818.2018.1517029
URL الوصول: https://doaj.org/article/2eee6bee21034d43b767618356a707bf
رقم الأكسشن: edsdoj.2eee6bee21034d43b767618356a707bf
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:13102818
13143530
76761835
DOI:10.1080/13102818.2018.1517029