دورية أكاديمية
Extracellular vesicles derived from M2‐like macrophages alleviate acute lung injury in a miR‐709‐mediated manner
| العنوان: | Extracellular vesicles derived from M2‐like macrophages alleviate acute lung injury in a miR‐709‐mediated manner |
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| المؤلفون: | Jie Yang, Xiaofang Huang, Qing Yu, Shibo Wang, Xuehuan Wen, Songjie Bai, Lanxin Cao, Kai Zhang, Shufang Zhang, Xingang Wang, Zhanghui Chen, Zhijian Cai, Gensheng Zhang |
| المصدر: | Journal of Extracellular Vesicles, Vol 13, Iss 4, Pp n/a-n/a (2024) |
| بيانات النشر: | Wiley, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | ALI/ARDS, alveolar macrophages, extracellular vesicles, M2 polarisation, miRNA, NLRP3 inflammasome, Cytology, QH573-671 |
| الوصف: | Abstract Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is characterised by an uncontrolled inflammatory response, and current treatment strategies have limited efficacy. Although the protective effect of M2‐like macrophages (M2φ) and their extracellular vesicles (EVs) has been well‐documented in other inflammatory diseases, the role of M2φ‐derived EVs (M2φ‐EVs) in the pathogenesis of ALI/ARDS remains poorly understood. The present study utilised a mouse model of lipopolysaccharide‐induced ALI to first demonstrate a decrease in endogenous M2‐like alveolar macrophage‐derived EVs. And then, intratracheal instillation of exogenous M2φ‐EVs from the mouse alveolar macrophage cell line (MH‐S) primarily led to a take up by alveolar macrophages, resulting in reduced lung inflammation and injury. Mechanistically, the M2φ‐EVs effectively suppressed the pyroptosis of alveolar macrophages and inhibited the release of excessive cytokines such as IL‐6, TNF‐α and IL‐1β both in vivo and in vitro, which were closely related to NF‐κB/NLRP3 signalling pathway inhibition. Of note, the protective effect of M2φ‐EVs was partly mediated by miR‐709, as evidenced by the inhibition of miR‐709 expression in M2φ‐EVs mitigated their protective effect against lipopolysaccharide‐induced ALI in mice. In addition, we found that the expression of miR‐709 in EVs derived from bronchoalveolar lavage fluid was correlated negatively with disease severity in ARDS patients, indicating its potential as a marker for ARDS severity. Altogether, our study revealed that M2φ‐EVs played a protective role in the pathogenesis of ALI/ARDS, partly mediated by miR‐709, offering a potential strategy for assessing disease severity and treating ALI/ARDS. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2001-3078 01644513 |
| Relation: | https://doaj.org/toc/2001-3078 |
| DOI: | 10.1002/jev2.12437 |
| URL الوصول: | https://doaj.org/article/d2f01644513d4b59ac449c1f8259aba5 |
| رقم الأكسشن: | edsdoj.2f01644513d4b59ac449c1f8259aba5 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20013078 01644513 |
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| DOI: | 10.1002/jev2.12437 |