دورية أكاديمية
Quantitative assessment of automated purification and concentration of E. coli bacteria
| العنوان: | Quantitative assessment of automated purification and concentration of E. coli bacteria |
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| المؤلفون: | Nina Sara Fraticelli Guzmán, Mohamed W. Badawy, Max A. Stockslager, Michael L. Farrell, Caitlin van Zyl, Seth Stewart, David L. Hu, Craig R. Forest |
| المصدر: | SLAS Technology, Vol 28, Iss 4, Pp 251-257 (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | Pathogen detection, Bacteria, Purification, Concentration, Filter, Automation, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Automated methods for rapidly purifying and concentrating bacteria from environmental interferents are needed in next-generation applications for anything from water purification to biological weapons detection. Though previous work has been performed by other researchers in this area, there is still a need to create an automated system that can both purify and concentrate target pathogens in a timely manner with readily available and replaceable components that could be easily integrated with a detection mechanism. Thus, the objective of this work was to design, build, and demonstrate the effectiveness of an automated system, the Automated Dual-filter method for Applied Recovery, or aDARE. aDARE uses a custom LABVIEW program that guides the flow of bacterial samples through a pair of size-based separation membranes to capture and elute the target bacteria. Using aDARE, we eliminated 95% of the interfering beads of a 5 mL-sample volume containing 107 CFU/mL of E. coli contaminated with 2 µm and 10 µm polystyrene beads at 106 beads/mL concentration., The target bacteria were concentrated to more than twice the initial concentration in 900 µL of eluent, resulting in an enrichment ratio for the target bacteria of 42 ± 13 in 5.5 min. These results show the feasibility and effectiveness of using size-based filtration membranes to purify and concentrate a target bacterium, in this case E. coli, in an automated system. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2472-6303 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2472630323000146; https://doaj.org/toc/2472-6303 |
| DOI: | 10.1016/j.slast.2023.02.004 |
| URL الوصول: | https://doaj.org/article/2f462b5b4e5d44ff8dcc0f3ee6293df0 |
| رقم الأكسشن: | edsdoj.2f462b5b4e5d44ff8dcc0f3ee6293df0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24726303 |
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| DOI: | 10.1016/j.slast.2023.02.004 |