دورية أكاديمية
THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate
| العنوان: | THOC5 complexes with DDX5, DDX17, and CDK12 to regulate R loop structures and transcription elongation rate |
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| المؤلفون: | Mareike Polenkowski, Aldrige Bernardus Allister, Sebastian Burbano de Lara, Andrew Pierce, Bethany Geary, Omar El Bounkari, Lutz Wiehlmann, Andrea Hoffmann, Anthony D. Whetton, Teruko Tamura, Doan Duy Hai Tran |
| المصدر: | iScience, Vol 26, Iss 1, Pp 105784- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | Molecular biology, Cell biology, Science |
| الوصف: | Summary: THOC5, a member of the THO complex, is essential for the 3′processing of some inducible genes, the export of a subset of mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3′cleavage of >50% of mRNAs and changes in RNA polymerase II binding across genes. THOC5 is recruited close to high-density polymerase II sites, suggesting that THOC5 is involved in transcriptional elongation. Indeed, measurement of elongation rates in vivo demonstrated decreased rates in THOC5-depleted cells. Furthermore, THOC5 is preferentially recruited to its target genes in slow polymerase II cells compared with fast polymerase II cells. Importantly chromatin-associated THOC5 interacts with CDK12 (a modulator of transcription elongation) and RNA helicases DDX5, DDX17, and THOC6 only in slow polymerase II cells. The CDK12/THOC5 interaction promotes CDK12 recruitment to R-loops in a THOC6-dependent manner. These data demonstrate a novel function of THOC5 in transcription elongation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2589-0042 42799228 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2589004222020570; https://doaj.org/toc/2589-0042 |
| DOI: | 10.1016/j.isci.2022.105784 |
| URL الوصول: | https://doaj.org/article/ea2f8fdeb28c42799228d559b21a2a93 |
| رقم الأكسشن: | edsdoj.2f8fdeb28c42799228d559b21a2a93 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25890042 42799228 |
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| DOI: | 10.1016/j.isci.2022.105784 |