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aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts

التفاصيل البيبلوغرافية
العنوان: aPKC-mediated displacement and actomyosin-mediated retention polarize Miranda in Drosophila neuroblasts
المؤلفون: Matthew Robert Hannaford, Anne Ramat, Nicolas Loyer, Jens Januschke
المصدر: eLife, Vol 7 (2018)
بيانات النشر: eLife Sciences Publications Ltd, 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
LCC:Biology (General)
مصطلحات موضوعية: polarity, asymmetric cell division, neuroblasts, Drosophila, Medicine, Science, Biology (General), QH301-705.5
الوصف: Cell fate assignment in the nervous system of vertebrates and invertebrates often hinges on the unequal distribution of molecules during progenitor cell division. We address asymmetric fate determinant localization in the developing Drosophila nervous system, specifically the control of the polarized distribution of the cell fate adapter protein Miranda. We reveal a step-wise polarization of Miranda in larval neuroblasts and find that Miranda’s dynamics and cortical association are differently regulated between interphase and mitosis. In interphase, Miranda binds to the plasma membrane. Then, before nuclear envelope breakdown, Miranda is phosphorylated by aPKC and displaced into the cytoplasm. This clearance is necessary for the subsequent establishment of asymmetric Miranda localization. After nuclear envelope breakdown, actomyosin activity is required to maintain Miranda asymmetry. Therefore, phosphorylation by aPKC and differential binding to the actomyosin network are required at distinct phases of the cell cycle to polarize fate determinant localization in neuroblasts.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2050-084X
Relation: https://elifesciences.org/articles/29939; https://doaj.org/toc/2050-084X
DOI: 10.7554/eLife.29939
URL الوصول: https://doaj.org/article/3077cca4c70e454ca319eb93ab9afd61
رقم الأكسشن: edsdoj.3077cca4c70e454ca319eb93ab9afd61
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:2050084X
DOI:10.7554/eLife.29939