دورية أكاديمية
Effects of bone types, particle sizes, and gamma irradiation doses in feline demineralized freeze-dried bone allograft
| العنوان: | Effects of bone types, particle sizes, and gamma irradiation doses in feline demineralized freeze-dried bone allograft |
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| المؤلفون: | Frizky Amelia, Basril Abbas, Darmawan Darwis, Sri Estuningsih, Deni Noviana |
| المصدر: | Veterinary World, Vol 13, Iss 8, Pp 1536-1543 (2020) |
| بيانات النشر: | Veterinary World, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | bone type, feline bone allograft, gamma irradiation, in vitro study, particle size, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: Fracture cases significantly increase recently, demanding high quality of bone graft materials. This research aimed to evaluate the effects of bone types, particle sizes, and gamma irradiation doses on morphological performance and cell viability of feline demineralized freeze-dried bone allograft (DFDBA) through an in vitro study. Materials and Methods: Feline DFDBA derived from feline cortical and cancellous long bones was processed into four different sizes: Group A (larger than 1000 μm), B (841-1000 μm), C (420-840 μm), and D (250-419 μm) for each type of bones. The materials were then irradiated with two doses of gamma rays, 15 and 25 kGy, resulting in 16 variants of feline DFDBA. The surfaces of each material were then observed with the scanning electron microscope (SEM). The in vitro evaluation of feline DFDBA was then performed using 3-(4,5-dimethythiazol-2)-2,5-diphenyltetrazolium bromide (MTT) assay with calf pulmonary artery endothelial cells. Results: The MTT assay results showed that the lowest inhibition rate (14.67±9.17 %) achieved by feline DFDBA in Group A derived from cortical bones irradiated with 15 kGy. Group D generally showed high inhibition rate in both cancellous and cortical bones, irradiated with either 15 or 25 kGy. The SEM results showed that cancellous and cortical bones have numerous macropores and micropores structure in 170× and 3000×, respectively. Conclusion: The material derived from cortical bones in Group A (larger than 1000 μm in particle size) irradiated with 15 kGy is the best candidate for further development due to its abundance of micropores structure and ability in preserving the living cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1536-1543 0972-8988 2231-0916 |
| Relation: | http://www.veterinaryworld.org/Vol.13/August-2020/7.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2020.1536-1543 |
| URL الوصول: | https://doaj.org/article/314482df259848248737470c3b932b5d |
| رقم الأكسشن: | edsdoj.314482df259848248737470c3b932b5d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15361543 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2020.1536-1543 |