دورية أكاديمية
Exosomes derived from tendon stem/progenitor cells enhance tendon-bone interface healing after rotator cuff repair in a rat model
| العنوان: | Exosomes derived from tendon stem/progenitor cells enhance tendon-bone interface healing after rotator cuff repair in a rat model |
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| المؤلفون: | Yanwei He, Shihao Lu, Wenbo Chen, Li Yang, Fangqi Li, Peng Zhou, Zan Chen, Renwen Wan, Zifan Zhang, Yaying Sun, Jinrong Lin, Yisheng Chen, Zhiwen Luo, Chen Xu, Shiyi Chen |
| المصدر: | Bioactive Materials, Vol 40, Iss , Pp 484-502 (2024) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| مصطلحات موضوعية: | Tendon stem cells, Exosome, Macrophages, MicroRNA-21a-5p, Rotator cuff tear, Tendon-to-bone healing, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| الوصف: | The rate of retear after surgical repair remains high. Mesenchymal stem cells (MSCs) have been extensively employed in regenerative medicine for several decades. However, safety and ethical concerns constrain their clinical application. Tendon Stem/Progenitor Cells (TSPCs)-derived exosomes have emerged as promising cell-free therapeutic agents. Therefore, urgent studies are needed to investigate whether TSPC-Exos could enhance tendon-bone healing and elucidate the underlying mechanisms. In this study, TSPC-Exos were found to promote the proliferation, migration, and expression of fibrogenesis markers in BMSCs. Furthermore, TSPC-Exos demonstrated an ability to suppress the polarization of M1 macrophages while promoting M2 macrophage polarization. In a rat model of rotator cuff repair, TSPC-Exos modulated inflammation and improved the histological structure of the tendon-bone interface, the biomechanical properties of the repaired tendon, and the function of the joint. Mechanistically, TSPC-Exos exhibited high expression of miR-21a-5p, which regulated the expression of PDCD4. The PDCD4/AKT/mTOR axis was implicated in the therapeutic effects of TSPC-Exos on proliferation, migration, and fibrogenesis in BMSCs. This study introduces a novel approach utilizing TSPC-Exos therapy as a promising strategy for cell-free therapies, potentially benefiting patients with rotator cuff tear in the future. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2452-199X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2452199X24002354; https://doaj.org/toc/2452-199X |
| DOI: | 10.1016/j.bioactmat.2024.06.014 |
| URL الوصول: | https://doaj.org/article/3184ffd99cba4e45ab834c9e5cc9e2ff |
| رقم الأكسشن: | edsdoj.3184ffd99cba4e45ab834c9e5cc9e2ff |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2452199X |
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| DOI: | 10.1016/j.bioactmat.2024.06.014 |