دورية أكاديمية
In vitro quality evaluation of leucocyte-reduced pooled platelet concentrates prepared from whole blood stored at 4℃
العنوان: | In vitro quality evaluation of leucocyte-reduced pooled platelet concentrates prepared from whole blood stored at 4℃ |
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المؤلفون: | Huijuan AN, Hong SHAN, Zheng LIU, Jiaojiao ZHANG, Jiaojie WANG, Lili BIE, Jianbin LI, Xinli JIN |
المصدر: | Zhongguo shuxue zazhi, Vol 37, Iss 7, Pp 820-826 (2024) |
بيانات النشر: | Institute of Blood Transfusion of Chinese Academy of Medical Sciences, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Diseases of the blood and blood-forming organs LCC:Medicine |
مصطلحات موضوعية: | leucocyte-reduced pooled platelet concentrates, whole blood cold storage, platelet quality, platelet function, Diseases of the blood and blood-forming organs, RC633-647.5, Medicine |
الوصف: | Objective To investigate the feasibility of leucocyte-reduced pooled platelet concentrates from whole blood stored at 4℃, and provide theoretical basis for the components preparation. Methods The collected 400 mL ACD-B anticoagulant whole blood was randomly divided into two groups, stored at 4℃ and room temperature. The buffy coat was prepared within 6 hours and store at 22℃ until next day to prepare leucocyte-reduced pooled platelet concentrates. Platelet samples on day 1, 3, 5 and 7 were taken for the blood cell count and related parameter detection. The pH, glucose and lactic acid content were determined to reflect the metabolic status, and the thromboelastography, platelet aggregation rate and PAC-1 and CD62P expression were determined to reflect the function and activation of platelets. The difference in platelets between two groups were analyzed. Results With the extension of storage time, the count of leucocyte-reduced pooled platelet concentrates decreased gradually, but the platelets distribution width (PDW), mean platelet volume (MPV) and platelet-larger cell ratio (P-LCR) increased gradually in two groups, with no statistical significance (P>0.05).The pH and glucose contents in two groups gradually decreased, but the lactic acid content gradually increased, with no significant difference (P>0.05). The thrombelastogram showed MA value that reflecting platelet function has no significant change during the storage, and there was no significant difference between the two groups (P>0.05). The aggregation rates decreased while the expression of PAC-1 and CD62P increased gradually with the prolongation of preservation time, with no significant difference between the two groups (P>0.05). Conclusion There is no significant difference in platelet count, function and activation between whole blood stored at 4℃ and at room temperature within 6 hours. Whole blood stored at 4℃ within 6 hours can be considered as the raw material for leucocyte-reduced pooled platelet concentrates. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | Chinese |
تدمد: | 1004-549X 1004-549x |
Relation: | https://www.cjbt.cn/thesisDetails#10.13303/j.cjbt.issn.1004-549x.2024.07.018&lang=en; https://doaj.org/toc/1004-549X |
DOI: | 10.13303/j.cjbt.issn.1004-549x.2024.07.018&lang=en |
DOI: | 10.13303/j.cjbt.issn.1004-549x.2024.07.018 |
URL الوصول: | https://doaj.org/article/318893810909423f96d9a31ced1c9b07 |
رقم الأكسشن: | edsdoj.318893810909423f96d9a31ced1c9b07 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 1004549X 1004549x |
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DOI: | 10.13303/j.cjbt.issn.1004-549x.2024.07.018&lang=en |