دورية أكاديمية
Pyroptosis in the lung and spleen of patients died from COVID-19
العنوان: | Pyroptosis in the lung and spleen of patients died from COVID-19 |
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المؤلفون: | Xin Tong, Haiqin Ping, Xiaoming Gong, Kai Zhang, Zhaojun Chen, Caiyun Cai, Zhiyan Lu, Rongrong Yang, Shicheng Gao, Yunyun Wang, Xinghuan Wang, Liang Liu, Hengning Ke |
المصدر: | European Journal of Inflammation, Vol 20 (2022) |
بيانات النشر: | SAGE Publishing, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Medicine |
مصطلحات موضوعية: | Medicine |
الوصف: | The purpose of this study was to investigate the expression of pyroptosis-related factors (NLRP3, IL-18, NF-κB, HMGB-1, and GSDMD) in patients who died of COVID-19. The expression levels of NLRP3, IL-18, NF-κB, HMGB-1, and GSDMD in lung and spleen tissues of the COVID-19 group and the control group were detected by tissue immunofluorescence. The control group includes lung tissues and spleen tissues of two patients who died unexpectedly without SARS-CoV-2 infection, and the COVID-19 group includes the lung and spleen tissues of three patients who died of SARS-CoV-2 virus infection. The positive rates of NF-κB, NLRP3, IL-18, and GSDMD in the lung tissues from the control group and COVID-19 group were 9.8% vs 73.4% ( p = 0.000), 5.5% vs 63.6% ( p = 0.000), 24.4% vs 76.2% ( p = 0.000), and 17.5% and 46.8% ( p = 0.000) respectively. The positive rates of NF-κB, NLRP3, IL-18, HMGB-1, and GSDMD in the spleen tissues from the control group and COVID-19 group were 20.6% vs 71.2% ( p = 0.000), 18.9% vs 72.0% ( p = 0.000), 15.2% vs 64.8% ( p = 0.000), 27.6% vs 69.2% ( p = 0.000), and 23% and 48.8% ( p = 0.000), respectively. The positive rates of SARS-CoV-2 spike protein in the CD68 positive cells of the lung and spleen in the control group and COVID-19 group were 2.5% vs 56.8% ( p = 0.000); 3.0% vs 64.9% ( p = 0.000) respectively. The rates of NF-κB positive nuclei in the control group and COVID-19 group were 13.4% vs 51.4% ( p = 0.000) in the lung and 38.2% vs 59.3% ( p = 0.000) in the spleen. The rates of HMGB-1 positive cytoplasm in the control and the COVID-19 group were 19.7% vs 50.3% ( p = 0.000) in the lung and 12.3% vs 45.2% ( p = 0.000) in the spleen. The targets of SARS-CoV-2 are the lung and spleen, where increased macrophages could be involved in the up-regulation of pyroptosis-related inflammatory factors such as NF-κB, HMGB-1, NLRP3, IL-18, and GSDMD. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2058-7392 1721727X |
Relation: | https://doaj.org/toc/2058-7392 |
DOI: | 10.1177/1721727X221140661 |
URL الوصول: | https://doaj.org/article/d31d0c3e7f464a39bd07c3588cdd9532 |
رقم الأكسشن: | edsdoj.31d0c3e7f464a39bd07c3588cdd9532 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20587392 1721727X |
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DOI: | 10.1177/1721727X221140661 |