دورية أكاديمية
Electronic Structure and Solvation Effects from Core and Valence Photoelectron Spectroscopy of Serum Albumin
| العنوان: | Electronic Structure and Solvation Effects from Core and Valence Photoelectron Spectroscopy of Serum Albumin |
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| المؤلفون: | Jean-Philippe Renault, Lucie Huart, Aleksandar R. Milosavljević, John D. Bozek, Jerôme Palaudoux, Jean-Michel Guigner, Laurent Marichal, Jocelyne Leroy, Frank Wien, Marie-Anne Hervé Du Penhoat, Christophe Nicolas |
| المصدر: | International Journal of Molecular Sciences, Vol 23, Iss 15, p 8227 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | X-ray electron spectroscopy, protein, electronic structure, theoretical chemistry, hydration, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | X-ray photoelectron spectroscopy of bovine serum albumin (BSA) in a liquid jet is used to investigate the electronic structure of a solvated protein, yielding insight into charge transfer mechanisms in biological systems in their natural environment. No structural damage was observed in BSA following X-ray photoelectron spectroscopy in a liquid jet sample environment. Carbon and nitrogen atoms in different chemical environments were resolved in the X-ray photoelectron spectra of both solid and solvated BSA. The calculations of charge distributions demonstrate the difficulty of assigning chemical contributions in complex systems in an aqueous environment. The high-resolution X-ray core electron spectra recorded are unchanged upon solvation. A comparison of the valence bands of BSA in both phases is also presented. These bands display a higher sensitivity to solvation effects. The ionization energy of the solvated BSA is determined at 5.7 ± 0.3 eV. Experimental results are compared with theoretical calculations to distinguish the contributions of various molecular components to the electronic structure. This comparison points towards the role of water in hole delocalization in proteins. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/23/15/8227; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms23158227 |
| URL الوصول: | https://doaj.org/article/e332d42394094fecb5f8e51488c99cb2 |
| رقم الأكسشن: | edsdoj.332d42394094fecb5f8e51488c99cb2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms23158227 |