دورية أكاديمية
A randomized in vitro investigation on the modulation of the TGF-β signaling pathway and induction of apoptosis in colorectal cancer cells by green seaweed Caulerpa racemosa
| العنوان: | A randomized in vitro investigation on the modulation of the TGF-β signaling pathway and induction of apoptosis in colorectal cancer cells by green seaweed Caulerpa racemosa |
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| المؤلفون: | Happy Kurnia Permatasari, Sarra Ben Bdira, Myunghan Moon, Nurlinah Amalia, Hikmawan Wahyu Sulistomo, Wibi Riawan, Jinwon Choi, Sanghyun Chung, Moon Nyeo Park, Byung-Kwan Seo, Fahrul Nurkolis, Bonglee Kim |
| المصدر: | Journal of Agriculture and Food Research, Vol 14, Iss , Pp 100796- (2023) |
| بيانات النشر: | Elsevier, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Agriculture (General) LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | Colorectal neoplasms, Transforming growth factor beta, Caulerpa, Antineoplastic agents, Apoptosis, Anticancer, Agriculture (General), S1-972, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | Colorectal cancer (CRC) poses a significant global health burden, necessitating innovative treatment strategies. This study evaluates the potential of Caulerpa racemosa extract, a natural compound, to modulate the transforming growth factor-beta (TGF-β) signaling pathway and induce apoptosis in CRC cells. HT-29 cells served as an in vitro CRC model. The cells were exposed to an n-hexane extract of C. racemosa, and TGF-β1 expression was assessed using immunofluorescence. Apoptosis was quantified via annexin V and propidium iodide staining through flow cytometry. C. racemosa extract significantly downregulated TGF-β1 expression compared to the control group, suggesting its potential as a CRC tumorigenesis and progression inhibitor. The extract displayed robust pro-apoptotic activity, with the 800 μg/mL dose significantly increasing early apoptotic cells compared to the 1200 μg/mL dose. Other dose comparisons showed no significant differences, indicating an optimal dosage range for early apoptosis induction at 800 μg/mL. The 1200 μg/mL dose also induced a notable increase in necrotic/late apoptotic cells compared to the control, triggering a more potent apoptotic response in advanced stages. Our study highlights the potential of C. racemosa extract to modulate the TGF-β signaling pathway and induce apoptosis in CRC cells. The observed dose-dependent effects on early and late apoptotic cells suggest potential applications as a preventive or therapeutic agent in CRC treatment. Further investigations are warranted to explore the extract's role in combatting CRC's complex pathogenesis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2666-1543 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2666154323003034; https://doaj.org/toc/2666-1543 |
| DOI: | 10.1016/j.jafr.2023.100796 |
| URL الوصول: | https://doaj.org/article/339cd4a463ce437c9af653aa324d2d71 |
| رقم الأكسشن: | edsdoj.339cd4a463ce437c9af653aa324d2d71 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 26661543 |
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| DOI: | 10.1016/j.jafr.2023.100796 |