دورية أكاديمية
Identification of potential immune-related mechanisms related to the development of multiple myeloma
العنوان: | Identification of potential immune-related mechanisms related to the development of multiple myeloma |
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المؤلفون: | Yaomei Wang, Wenli Zhang, Tiandong Li, Mengmeng Liu, Mengya Gao, Xinqing Li, Yufei Chen, Yongping Song, Wei Li, Chunyan Du, Fang Wang, Lina Liu, Sihan Zhou, Xiuyuan Hao |
المصدر: | Chinese Medical Journal, Vol 137, Iss 13, Pp 1603-1613 (2024) |
بيانات النشر: | Wolters Kluwer, 2024. |
سنة النشر: | 2024 |
المجموعة: | LCC:Medicine |
مصطلحات موضوعية: | Medicine |
الوصف: | Abstract. Background:. Although significant advances have been made in the treatment of multiple myeloma (MM), leading to unprecedented response and survival rates among patients, the majority eventually relapse, and a cure remains elusive. This situation is closely related to an incomplete understanding of the immune microenvironment, especially monocytes/macrophages in patients with treatment-naïve MM. The aim of this study was to provide insight into the immune microenvironment, especially monocytes/macrophages, in patients with treatment-naïve MM. Methods:. This study used the single-cell RNA sequencing (scRNA-seq) data of both patients with MM and heathy donors to identify immune cells, including natural killer (NK) cells, T cells, dendritic cells (DCs), and monocytes/macrophages. Transcriptomic data and flow cytometry analysis of monocytes/macrophages were used to further examine the effect of monocytes/macrophages in treatment-naïve MM patients. Results:. A significant difference was observed between the bone marrow (BM) immune cells of the healthy controls and treatment-naïve MM patients through scRNA-seq. It is noteworthy that, through an scRNA-seq data analysis, this study found that interferon (IFN)-induced NK/T cells, terminally differentiated effector memory (TEMRA) cells, T-helper cells characterized by expression of IFN-stimulated genes (ISG+Th cells), IFN-responding exhausted T cells, mannose receptor C-type 1 (MRC1)+ DCs, IFN-responding DCs, MHCII+ DCs, and immunosuppressive monocytes/macrophages were enriched in patients with treatment-naïve MM. Significantly, transcriptomic data of monocytes/macrophages demonstrated that “don’t eat me”-related genes and IFN-induced genes increase in treatment-naïve MM patients. Furthermore, scRNA-seq, transcriptomic data, and flow cytometry also showed an increased proportion of CD16+ monocytes/macrophages and expression level of CD16. Cell–cell communication analysis indicated that monocytes/macrophages, whose related important signaling pathways include migration inhibitory factor (MIF) and interleukin 16 (IL-16) signaling pathway, are key players in treatment-naïve MM patients. Conclusions:. Our findings provide a comprehensive and in-depth molecular characterization of BM immune cell census in MM patients, especially for monocytes/macrophages. Targeting macrophages may be a novel treatment strategy for patients with MM. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 0366-6999 2542-5641 00000000 |
Relation: | http://journals.lww.com/10.1097/CM9.0000000000003116; https://doaj.org/toc/0366-6999; https://doaj.org/toc/2542-5641 |
DOI: | 10.1097/CM9.0000000000003116 |
URL الوصول: | https://doaj.org/article/d33f19cce9f14844801e95cf1e3f9bad |
رقم الأكسشن: | edsdoj.33f19cce9f14844801e95cf1e3f9bad |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 03666999 25425641 00000000 |
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DOI: | 10.1097/CM9.0000000000003116 |