دورية أكاديمية
أعرض في EDS

Expression profile analysis of LncRNAs and mRNAs in pre-receptive endometrium of women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer

التفاصيل البيبلوغرافية
العنوان: Expression profile analysis of LncRNAs and mRNAs in pre-receptive endometrium of women with polycystic ovary syndrome undergoing in vitro fertilization-embryo transfer
المؤلفون: Xiuhua Xu, Aimin Yang, Pengxiang Tian, Kun Zhang, Yuanyuan Liu, Yizhuo Wang, Ziwei Wang, Yanjing Wu, Zhiming Zhao, Qian Li, Baojun shi, Xianghua Huang, Gui-min Hao
المصدر: BMC Medical Genomics, Vol 17, Iss 1, Pp 1-14 (2024)
بيانات النشر: BMC, 2024.
سنة النشر: 2024
المجموعة: LCC:Internal medicine
LCC:Genetics
مصطلحات موضوعية: PCOS, Pre-receptive endometrium, Endometrial receptivity, lncRNA, lncRNA-mRNA network, Internal medicine, RC31-1245, Genetics, QH426-470
الوصف: Abstract Background To compare the expression levels of long non-coding RNA (lncRNA) and messenger RNA (mRNA) in pre-receptive endometrium between patients with Polycystic Ovary Syndrome (PCOS)and normal ovulation undergoing in vitro fertilization-embryo transfer (IVF-ET). Methods Endometrial tissues were collected with endometrial vacuum curette in pre-receptive phase (3 days after oocytes retrieval) from PCOS and control groups. LncRNAs and mRNAs of endometrium were identified via RNA sequencing and alignments. A subset of 9 differentially expressed lncRNAs and 11 mRNAs were validated by quantitative reverse transcription polymerase chain reaction(qRT-PCR)in 22 PCOS patients and 18 ovulation patients. The function of mRNAs with differential expression patterns were explored using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results We found out 687 up-regulated and 680 down-regulated mRNAs, as well as 345 up-regulated and 63 down-regulated lncRNAs in the PCOS patients in contrast to normal ovulation patients. qRT-PCR was used to detect the expression of 11 mRNAs, and validated that the expression of these 6 mRNAs CXCR4, RABL6, OPN3, SYBU, IDH1, NOP10 were significantly elevated among PCOS patients, and the expression of ZEB1 was significantly decreased. qRT-PCR was performed to detect the expression of 9 lncRNAs, and validated that the expression of these 7 lncRNAs IDH1-AS1, PCAT14, FTX, DANCR, PRKCQ-AS1, SNHG8, TPT1-AS1 were significantly enhanced among PCOS patients. Bioinformatics analysis showed that differentially expressed genes (DEGs) involved KEGG pathway were tyrosine metabolism, PI3K-Akt pathway, metabolic pathway, Jak-STAT pathway, pyruvate metabolism, protein processing in endoplasmic reticulum, oxidative phosphorylation and proteasome. The up-regulation of GO classification was involved in ATP metabolic process, oxidative phosphorylation, RNA catabolic process, and down-regulation of GO classification was response to corticosteroid, steroid hormone, and T cell activation. Conclusion Our results determined the characteristics and expression profile of endometrial lncRNAs and mRNAs in PCOS patients in pre-receptive phase, which is the day 3 after oocytes retrival. The possible pathways and related genes of endometrial receptivity disorders were found, and those lncRNAs may be developed as a predictive biomarker of endometrium in pre-receptive phase.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1755-8794
Relation: https://doaj.org/toc/1755-8794
DOI: 10.1186/s12920-024-01806-w
URL الوصول: https://doaj.org/article/343227139b7a466a80af60581f324c8e
رقم الأكسشن: edsdoj.343227139b7a466a80af60581f324c8e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17558794
DOI:10.1186/s12920-024-01806-w