دورية أكاديمية
أعرض في EDS

Single-cell RNA-sequencing reveals distinct immune cell subsets and signaling pathways in IgA nephropathy

التفاصيل البيبلوغرافية
العنوان: Single-cell RNA-sequencing reveals distinct immune cell subsets and signaling pathways in IgA nephropathy
المؤلفون: Honghui Zeng, Le Wang, Jiajia Li, Siweier Luo, Qianqian Han, Fang Su, Jing Wei, Xiaona Wei, Jianping Wu, Bin Li, Jingang Huang, Patrick Tang, Chunwei Cao, Yiming Zhou, Qiongqiong Yang
المصدر: Cell & Bioscience, Vol 11, Iss 1, Pp 1-21 (2021)
بيانات النشر: BMC, 2021.
سنة النشر: 2021
المجموعة: LCC:Biotechnology
LCC:Biology (General)
LCC:Biochemistry
مصطلحات موضوعية: IgA nephropathy, Single-cell RNA seq, Immune cell landscape, Natural killer cells, Monocytes, B cells, Biotechnology, TP248.13-248.65, Biology (General), QH301-705.5, Biochemistry, QD415-436
الوصف: Abstract Background IgA nephropathy (IgAN) is the most common primary glomerulonephritis globally. Increasing evidence suggests the importance of host immunity in the development of IgAN, but its dynamics during the early stage of IgAN are still largely unclear. Results Here we successfully resolved the early transcriptomic changes in immune cells of IgAN by conducting single-cell RNA-sequencing (scRNA-seq) with peripheral blood mononuclear cells. The differentially expressed genes (DEGs) between control and IgAN were predominantly enriched in NK cell-mediated cytotoxicity and cell killing pathways. Interestingly, we discovered that the number and cytotoxicity of NK cells are significantly reduced in IgAN patients, where both the number and marker genes of NK cells were negatively associated with the clinical parameters, including the levels of urine protein creatinine ratio (UPCR), serum galactose-deficient IgA1 and IgA. A distinctive B cell subset, which had suppressed NFκB signaling was predominantly in IgAN and positively associated with disease progression. Moreover, the DEGs of B cells were enriched in different viral infection pathways. Classical monocytes also significantly changed in IgAN and a monocyte subset expressing interferon-induced genes was positively associated with the clinical severity of IgAN. Finally, we identified vast dynamics in intercellular communications in IgAN. Conclusions We dissected the immune landscape of IgAN at the single-cell resolution, which provides new insights in developing novel biomarkers and immunotherapy against glomerulonephritis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-3701
Relation: https://doaj.org/toc/2045-3701
DOI: 10.1186/s13578-021-00706-1
URL الوصول: https://doaj.org/article/3442d1b1bf6944a7ae4968b62c3796fe
رقم الأكسشن: edsdoj.3442d1b1bf6944a7ae4968b62c3796fe
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20453701
DOI:10.1186/s13578-021-00706-1