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Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates

التفاصيل البيبلوغرافية
العنوان: Tissue distribution and cell tropism of Brucella canis in naturally infected canine foetuses and neonates
المؤلفون: Tayse Domingues de Souza, Tatiane Furtado de Carvalho, Juliana Pinto da Silva Mol, João Vítor Menezes Lopes, Monique Ferreira Silva, Tatiane Alves da Paixão, Renato Lima Santos
المصدر: Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
بيانات النشر: Nature Portfolio, 2018.
سنة النشر: 2018
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Abstract Brucella canis infection is an underdiagnosed zoonotic disease. Knowledge about perinatal brucellosis in dogs is extremely limited, although foetuses and neonates are under risk of infection due to vertical transmission. In this study, immunohistochemistry was used to determine tissue distribution and cell tropism of B. canis in canine foetuses and neonates. Diagnosis of B. canis in tissues of naturally infected pups was based on PCR and sequencing of amplicons, bacterial isolation, and immunohistochemistry, whose specificity was confirmed by laser capture microdissection. PCR positivity among 200 puppies was 21%, and nine isolates of B. canis were obtained. Tissues from 13 PCR-positive puppies (4 stillborn and 9 neonates) presented widespread immunolabeling. Stomach, intestines, kidney, nervous system, and umbilicus were positive in all animals tested. Other frequently infected organs included the liver (92%), lungs (85%), lymph nodes (69%), and spleen (62%). Immunolabeled coccobacilli occurred mostly in macrophages, but they were also observed in erythrocytes, epithelial cells of gastrointestinal mucosa, renal tubules, epidermis, adipocytes, choroid plexus, ependyma, neuroblasts, blood vessels endothelium, muscle cells, and in the intestinal lumen. These results largely expand our knowledge about perinatal brucellosis in the dog, clearly demonstrating a pantropic distribution of B. canis in naturally infected foetuses and neonates.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
Relation: https://doaj.org/toc/2045-2322
DOI: 10.1038/s41598-018-25651-x
URL الوصول: https://doaj.org/article/349ea1a0071d40828628eaa2a49dac69
رقم الأكسشن: edsdoj.349ea1a0071d40828628eaa2a49dac69
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-018-25651-x