دورية أكاديمية
Injectable kartogenin and apocynin loaded micelle enhances the alleviation of intervertebral disc degeneration by adipose-derived stem cell
| العنوان: | Injectable kartogenin and apocynin loaded micelle enhances the alleviation of intervertebral disc degeneration by adipose-derived stem cell |
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| المؤلفون: | Chao Yu, Dongdong Li, Chenggui Wang, Kaishun Xia, Jingkai Wang, Xiaopeng Zhou, Liwei Ying, Jiawei Shu, Xianpeng Huang, Haibin Xu, Bin Han, Qixin Chen, Fangcai Li, Jianbin Tang, Chengzhen Liang, Nigel Slater |
| المصدر: | Bioactive Materials, Vol 6, Iss 10, Pp 3568-3579 (2021) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| مصطلحات موضوعية: | Intervertebral disc degeneration, Polymer-drug conjugates, Mesenchymal stem cell, Reactive oxygen species, Stem cell therapy, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| الوصف: | Cell transplantation has been proved the promising therapeutic effects on intervertebral disc degeneration (IVDD). However, the increased levels of reactive oxygen species (ROS) in the degenerated region will impede the efficiency of human adipose-derived stem cells (human ADSCs) transplantation therapy. It inhibits human ADSCs proliferation, and increases human ADSCs apoptosis. Herein, we firstly devised a novel amphiphilic copolymer PEG-PAPO, which could self-assemble into a nanosized micelle and load lipophilic kartogenin (KGN), as a single complex (PAKM). It was an injectable esterase-responsive micelle, and showed controlled release ability of KGN and apocynin (APO). Oxidative stimulation promoted the esterase activity in human ADSCs, which accelerate degradation of esterase-responsive micelle. Compared its monomer, the PAKM micelle possessed better bioactivities, which were attributed to their synergistic effect. It enhanced the viability, autophagic activation (P62, LC3 II), ECM-related transcription factor (SOX9), and ECM (Collagen II, Aggrecan) maintenance in human ADSCs. Furthermore, it is demonstrated that the injection of PAKM with human ADSCs yielded higher disc height and water content in rats. Therefore, PAKM micelles perform promoting cell survival and differentiation effects, and may be a potential therapeutic agent for IVDD. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2452-199X |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2452199X21001298; https://doaj.org/toc/2452-199X |
| DOI: | 10.1016/j.bioactmat.2021.03.018 |
| URL الوصول: | https://doaj.org/article/34ec63e8de864c3ab1353cf746a83b3a |
| رقم الأكسشن: | edsdoj.34ec63e8de864c3ab1353cf746a83b3a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2452199X |
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| DOI: | 10.1016/j.bioactmat.2021.03.018 |