دورية أكاديمية
A bypass flow model to study endothelial cell mechanotransduction across diverse flow environments
| العنوان: | A bypass flow model to study endothelial cell mechanotransduction across diverse flow environments |
|---|---|
| المؤلفون: | Zhuotao Xiao, Rudmer J. Postma, Anton Jan van Zonneveld, Bernard M. van den Berg, Wendy M.P.J. Sol, Nicholas A. White, Huybert J.F. van de Stadt, Asad Mirza, Jun Wen, Roel Bijkerk, Joris I. Rotmans |
| المصدر: | Materials Today Bio, Vol 27, Iss , Pp 101121- (2024) |
| بيانات النشر: | Elsevier, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Disturbed flow, In vitro model, Vascular bifurcation, Computational fluid dynamics, High-content screening, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Disturbed flow is one of the pathological initiators of endothelial dysfunction in intimal hyperplasia (IH) which is commonly seen in vascular bypass grafts, and arteriovenous fistulas. Various in vitro disease models have been designed to simulate the hemodynamic conditions found in the vasculature. Nonetheless, prior investigations have encountered challenges in establishing a robust disturbed flow model, primarily attributed to the complex bifurcated geometries and distinctive flow dynamics. In the present study, we aim to address this gap by introducing an in vitro bypass flow model capable of inducing disturbed flow and other hemodynamics patterns through a pulsatile flow in the same model. To assess the model's validity, we employed computational fluid dynamics (CFD) to simulate hemodynamics and compared the morphology and functions of human umbilical venous endothelial cells (HUVECs) under disturbed flow conditions to those in physiological flow or stagnant conditions. CFD analysis revealed the generation of disturbed flow within the model, pinpointing the specific location in the channel where the effects of disturbed flow were observed.High-content screening, a single-cell morphological profile assessment, demonstrated that HUVECs in the disturbed flow area exhibited random orientation, and morphological features were significantly distinct compared to cells in the physiological flow or stagnant condition after a two days of flow exposure. Furthermore, HUVECs exposed to disturbed flow underwent extensive remodeling of the adherens junctions and expressed higher levels of endothelial cell activation markers compared to other hemodynamic conditions.In conclusion, our in vitro bypass flow model provides a robust platform for investigating the associations between disturbed flow pattern and vascular diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2590-0064 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2590006424001807; https://doaj.org/toc/2590-0064 |
| DOI: | 10.1016/j.mtbio.2024.101121 |
| URL الوصول: | https://doaj.org/article/355f4da060c2491ebb47f7b3e71599bc |
| رقم الأكسشن: | edsdoj.355f4da060c2491ebb47f7b3e71599bc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25900064 |
|---|---|
| DOI: | 10.1016/j.mtbio.2024.101121 |