We present the first records of Tropidurus chromatops Harvey & Gutberlet, 1998 from the Beni Department (Bolivia) and extend this species’ distribution to western Bolivia by approximately 256 km in a straight line to the Beni Floodplains ecoregion. Likewise, we contribute information on some aspects of the natural history, altitude of occurrence, and the geographic distribution of T. chromatops in the Department of Beni.