دورية أكاديمية
Development of inducer-free expression plasmids using IPTG-inducible Pspac promoter for Bacillus subtilis
| العنوان: | Development of inducer-free expression plasmids using IPTG-inducible Pspac promoter for Bacillus subtilis |
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| المؤلفون: | Phuong Thi Bich Chu, Hanh Thi Thu Phan, Hoang Duc Nguyen, Trang Thi Phuong Phan |
| المصدر: | Vietnam Journal of Science, Technology and Engineering, Vol 63, Iss 1 (2021) |
| بيانات النشر: | Vietnam Ministry of Science and Technology, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | Bacillus subtilis, inducer-free, pHT vector, Pspac, weak promoter, Science |
| الوصف: | An inducer-free expression vector for the low expression levels in Bacillus subtilis (B. subtilis) is necessary for fundamental research. In this study, we constructed inducer-free expression plasmids carrying Pspac, a well-known IPTG-inducible promoter, by removing a part of the lacI gene. Then, we analysed the expression of the target genes bgaB and gfp+ in B. subtilis. Western blot experiments demonstrated that the reporters from the inducer-free plasmids with Pspac could be produced at low levels in B. subtilis strains and were equivalent to their corresponding inducible constructs with 1 mM IPTG. The reporter activities showed that inducer-free expression from the Pspac promoter was dramatically less than that of the inducer-free plasmids with the strong promoters Pgrac01 and Pgrac100 reported previously, about 16.2 to 20.3 times for BgaB and 24.7 to 34.3 times for GFP+, respectively. In conclusion, the inducer-free expression vectors carrying Pspac promoters allow the constitutive expression of heterologous recombinant proteins at low levels in B. subtilis. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2525-2461 2615-9937 |
| Relation: | https://vietnamscience.vjst.vn/index.php/vjste/article/view/22; https://doaj.org/toc/2525-2461; https://doaj.org/toc/2615-9937 |
| DOI: | 10.31276/VJSTE.63(1).64-70 |
| URL الوصول: | https://doaj.org/article/a3607a7d318f431db1cf2223a89a12f7 |
| رقم الأكسشن: | edsdoj.3607a7d318f431db1cf2223a89a12f7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25252461 26159937 |
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| DOI: | 10.31276/VJSTE.63(1).64-70 |