دورية أكاديمية
Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway
| العنوان: | Propofol pretreatment attenuates lipopolysaccharide-induced acute lung injury in rats by activating the phosphoinositide-3-kinase/Akt pathway |
|---|---|
| المؤلفون: | L.L. Zhao, G.C. Hu, S.S. Zhu, J.F. Li, G.J. Liu |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 47, Iss 12, Pp 1062-1067 (2014) |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Acute lung injury, Propofol, Lipopolysaccharide, PI3K/Akt pathway, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | The aim of this study was to investigate the effect of propofol pretreatment on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and the role of the phosphoinositide-3-kinase/protein kinase B (PI3K/Akt) pathway in this procedure. Survival was determined 48 h after LPS injection. At 1 h after LPS challenge, the lung wet- to dry-weight ratio was examined, and concentrations of protein, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) were determined using the bicinchoninic acid method or ELISA. Lung injury was assayed via lung histological examination. PI3K and p-Akt expression levels in the lung tissue were determined by Western blotting. Propofol pretreatment prolonged survival, decreased the concentrations of protein, TNF-α, and IL-6 in BALF, attenuated ALI, and increased PI3K and p-Akt expression in the lung tissue of LPS-challenged rats, whereas treatment with wortmannin, a PI3K/Akt pathway specific inhibitor, blunted this effect. Our study indicates that propofol pretreatment attenuated LPS-induced ALI, partly by activation of the PI3K/Akt pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1414-431X |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201062&lng=en&tlng=en; https://doaj.org/toc/1414-431X |
| DOI: | 10.1590/1414-431X20143949 |
| URL الوصول: | https://doaj.org/article/367dbc0872b44190a62f4e5f417fd854 |
| رقم الأكسشن: | edsdoj.367dbc0872b44190a62f4e5f417fd854 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1414431X |
|---|---|
| DOI: | 10.1590/1414-431X20143949 |