دورية أكاديمية
Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro
| العنوان: | Pengaruh Umur Biak dan Posisi Daun terhadap Morfogenesis dari Daun Kentang Hitam (Solenostemon rotundifolius (POIR) JK Morton In Vitro |
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| المؤلفون: | Tatin Suherlina, Aryani Leksonowati, Witjaksono Witjaksono |
| المصدر: | Journal of Biological Researches, Vol 17, Iss 1, Pp 97-101 (2012) |
| بيانات النشر: | Indonesian Biological Society, 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | in vitro culture, leaf position, organogenesis, Solenostemon rotundifolius (Poir) JK Morton, Culture age, In vitro culture, Leaf position, Organogenesis, Science, Biology (General), QH301-705.5 |
| الوصف: | Plant regeneration via somatic embryogenesis or organogenesis is a key for manipulation of somatic cells in vitro. Organogenesis from leaf inoculum, petiole and stem segments of in vitro black potato (Solenostemon rotundifolius (Poir) JK Morton) is influenced by the balance of the concentration of BA and NAA in the culture medium. However, organogenesis could also be affected by factors inherent to the inoculums. This paper reports the influence of leaf position on the in vitro shoots and culture age on the formation and production of shoot adventives from leaf inoculum. Leaves excised from shoots of 3-8 week-old culture and leaf position 1-5 from apical to basal was used as inocula. The highest percentage (83.3%) of adventitious bud develops at cultures age of 5 weeks with the average number of shoots 6.1. The highest percentage of bud development occurs at leaf position number 2-4, with the percentage of buds of 83.3, 76.2 and 76.2, respectively with the average number of shoots of 3.19, 2.10 dan 2.38 respectively. Organogenesis that produces the highest shoot production of 18.6 per Petri dish was obtained from leaves derived from the 2ndleaf from apical of 5 week-old culture inoculated on MS medium enriched with 5 mg/l BA and 0.1 mg/l NAA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Indonesian |
| تدمد: | 0852-6834 2337-389X |
| Relation: | http://berkalahayati.org/journal/cc138f8d; https://doaj.org/toc/0852-6834; https://doaj.org/toc/2337-389X |
| DOI: | 10.23869/bphjbr.17.1.201119 |
| URL الوصول: | https://doaj.org/article/c3782f89b0954d84a1e658f029559415 |
| رقم الأكسشن: | edsdoj.3782f89b0954d84a1e658f029559415 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 08526834 2337389X |
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| DOI: | 10.23869/bphjbr.17.1.201119 |