دورية أكاديمية
Functional comparison of distinct Brachyury+ states in a renal differentiation assay
| العنوان: | Functional comparison of distinct Brachyury+ states in a renal differentiation assay |
|---|---|
| المؤلفون: | Jing Zhou, Antonius Plagge, Patricia Murray |
| المصدر: | Biology Open, Vol 7, Iss 5 (2018) |
| بيانات النشر: | The Company of Biologists, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | Mouse embryonic stem cells, Embryoid bodies, Brachyury, Mesodermal differentiation, Ex vivo culture, Kidney rudiments, Science, Biology (General), QH301-705.5 |
| الوصف: | Mesodermal populations can be generated in vitro from mouse embryonic stem cells (mESCs) using three-dimensional (3-D) aggregates called embryoid bodies or two-dimensional (2-D) monolayer culture systems. Here, we investigated whether Brachyury-expressing mesodermal cells generated using 3-D or 2-D culture systems are equivalent or, instead, have different properties. Using a Brachyury-GFP/E2-Crimson reporter mESC line, we isolated Brachyury-GFP+ mesoderm cells using flow-activated cell sorting and compared their gene expression profiles and ex vivo differentiation patterns. Quantitative real-time polymerase chain reaction analysis showed significant up-regulation of Cdx2, Foxf1 and Hoxb1 in the Brachyury-GFP+ cells isolated from the 3-D system compared with those isolated from the 2-D system. Furthermore, using an ex vivo mouse kidney rudiment assay, we found that, irrespective of their source, Brachyury-GFP+ cells failed to integrate into developing nephrons, which are derived from the intermediate mesoderm. However, Brachyury-GFP+ cells isolated under 3-D conditions appeared to differentiate into endothelial-like cells within the kidney rudiments, whereas the Brachyury-GFP+ isolated from the 2-D conditions only did so to a limited degree. The high expression of Foxf1 in the 3-D Brachyury-GFP+ cells combined with their tendency to differentiate into endothelial-like cells suggests that these mesodermal cells may represent lateral plate mesoderm. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-6390 |
| Relation: | http://bio.biologists.org/content/7/5/bio031799; https://doaj.org/toc/2046-6390 |
| DOI: | 10.1242/bio.031799 |
| URL الوصول: | https://doaj.org/article/37f2e60971d8465f9165a1208f49fe9a |
| رقم الأكسشن: | edsdoj.37f2e60971d8465f9165a1208f49fe9a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20466390 |
|---|---|
| DOI: | 10.1242/bio.031799 |