دورية أكاديمية
Expression of Phospholipase D Family Member 6 in Bovine Testes and Its Molecular Characteristics
| العنوان: | Expression of Phospholipase D Family Member 6 in Bovine Testes and Its Molecular Characteristics |
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| المؤلفون: | Rui Yang, Boyang Zhang, Wenqian Zhu, Chunling Zhu, Lanxin Chen, Yansen Zhao, Yueqi Wang, Yan Zhang, Amjad Riaz, Bo Tang, Xueming Zhang |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 15, p 12172 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | bovine, bioinformatics, phospholipase D family member 6, testes, spermatogonial stem cells, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Spermatogonial stem cells (SSCs) are the only primitive spermatogonial cells in males that can naturally transmit genetic information to their offspring and replicate throughout their lives. Phospholipase D family member 6 (PLD6) has recently been found to be a surface marker for SSCs in mice and boars; however, it has not been validated in cattle. The results of reversed transcription-polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qRT-PCR) found that the relative expression of the PLD6 gene in the testicular tissues of two-year-old Simmental calves was significantly higher than that of six-month-old calves. Immunofluorescent staining further verified the expression of PLD6 protein in bovine spermatogenic cells like germ cell marker DEAD box helicase 4 (DDX4, also known as VASA). Based on multiple bioinformatic databases, PLD6 is a conservative protein which has high homology with mouse Q5SWZ9 protein. It is closely involved in the normal functioning of the reproductive system. Molecular dynamics simulation analyzed the binding of PLD6 as a phospholipase to cardiolipin (CL), and the PLD6-CL complex showed high stability. The protein interaction network analysis showed that there is a significant relationship between PLD6 and piwi-interacting RNA (piRNA) binding protein. PLD6 acts as an endonuclease and participates in piRNA production. In addition, PLD6 in bovine and mouse testes has a similar expression pattern with the spermatogonium-related genes VASA and piwi like RNA-mediated gene silencing 2 (PIWIL2). In conclusion, these analyses imply that PLD6 has a relatively high expression in bovine testes and could be used as a biomarker for spermatogenic cells including SSCs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/15/12172; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms241512172 |
| URL الوصول: | https://doaj.org/article/380e0e69f8274ef3b14c5a44c5f8a282 |
| رقم الأكسشن: | edsdoj.380e0e69f8274ef3b14c5a44c5f8a282 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms241512172 |