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Molecular characterization of several Wx alleles in durum wheat

التفاصيل البيبلوغرافية
العنوان: Molecular characterization of several Wx alleles in durum wheat
المؤلفون: R. Ortega, C. Guzmán, J. B. Alvarez
المصدر: Biologia Plantarum, Vol 59, Iss 2, Pp 220-226 (2015)
بيانات النشر: Institute of Experimental Botany of the Czech Academy of Sciences, 2015.
سنة النشر: 2015
المجموعة: LCC:Biology (General)
LCC:Plant ecology
مصطلحات موضوعية: pcr amplification, sds-page, triticum turgidum, waxy proteins, Biology (General), QH301-705.5, Plant ecology, QK900-989
الوصف: The Wx gene, which encodes waxy proteins, is the sole gene responsible for amylose synthesis in the wheat seed endosperm. In this study, we characterized, at the molecular level, several Wx alleles in durum wheat (Triticum turgidum L. ssp. durum Desf.) that had previously been catalogued at the protein level. Our data show a misclassification of the alleles in both Wx genes: Wx-A1 alleles from Blanqueta (Wx-A1a) and Astrodur (Wx-A1b) have been reclassified as Wx-A1a and Wx-A1h, respectively. A sequence comparison of the Wx-B1e allele shows that there were up to five different alleles under this denomination, which confirms that the protein analysis by SDS-PAGE separation needs to be combined with the PCR amplification-sequencing analysis in order to obtain a correct classification of the alleles, which will facilitate their use in wheat quality improvement.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1573-8264
Relation: https://bp.ueb.cas.cz/artkey/bpl-201502-0003_Molecular-characterization-of-several-Wx-alleles-in-durum-wheat.php; https://doaj.org/toc/1573-8264
DOI: 10.1007/s10535-015-0507-0
URL الوصول: https://doaj.org/article/381b392031034972a4bdc76643917f3c
رقم الأكسشن: edsdoj.381b392031034972a4bdc76643917f3c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:15738264
DOI:10.1007/s10535-015-0507-0