دورية أكاديمية
SIS3 alleviates cisplatin-induced acute kidney injury by regulating the lncRNA Arid2-IR-transferrin receptor pathway
| العنوان: | SIS3 alleviates cisplatin-induced acute kidney injury by regulating the lncRNA Arid2-IR-transferrin receptor pathway |
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| المؤلفون: | Jiayan Huang, Weiyan Lai, Ming Li, Canming Li, Tanqi Lou, Hui Peng, Zengchun Ye |
| المصدر: | Kidney & Blood Pressure Research (2022) |
| بيانات النشر: | Karger Publishers, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Dermatology LCC:Diseases of the circulatory (Cardiovascular) system LCC:Diseases of the genitourinary system. Urology |
| مصطلحات موضوعية: | Dermatology, RL1-803, Diseases of the circulatory (Cardiovascular) system, RC666-701, Diseases of the genitourinary system. Urology, RC870-923 |
| الوصف: | Introduction: TGF-β/Smad3 may be involved in the pathogenesis of acute kidney injury (AKI), but its functional role and mechanism of action in cisplatin-induced AKI are unclear. Here, we established a cisplatin-induced AKI mouse model to demonstrate that Smad3 may have roles in cisplatin nephropathy because of its potential effects on tubular epithelial cell (TEC) death and regeneration. Methods: Using a cisplatin-induced AKI model, the expression levels of lncRNA Arid2-IR were measured by qRT-PCR and the location detected by FISH. Transfected with overexpression lncRNA Arid2-IR by lentiviral vector in TECs, and the expression of Cleaved Caspase-3, Bax, Bcl-2, PCNA, P21, P27, TFRC, FTH and FTL were measured by western blot. Protein molecules bound to lncRNA Arid2-IR were identified by RIP, RNA pull-down assay, mass spectrometry. Results：LncRNA Arid2-IR was significantly downregulated in vivo and in vitro. SIS3 decreased cell apoptosis and promoted cell regeneration by upregulating lncRNA Arid2-IR expression. LncRNA Arid2-IR regulated the cell cycle by decreasing expression of the cyclin dependent kinase inhibitors P21 and P27. Finally, lncRNA Arid2-IR interacted with the transferrin receptor (TFRC), overexpression of lncRNA Arid2-IR increased TFRC expression and decreased FTH and FTL. Conclusion：Smad3 regulated lncRNA Arid2-IR via TFRC, thereby regulating the cell cycle, protecting against cell apoptosis and promoting cell regeneration. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1420-4096 1423-0143 |
| Relation: | https://www.karger.com/Article/FullText/527713; https://doaj.org/toc/1420-4096; https://doaj.org/toc/1423-0143 |
| DOI: | 10.1159/000527713 |
| URL الوصول: | https://doaj.org/article/aa3846dff6104c62baf1b598edc29332 |
| رقم الأكسشن: | edsdoj.3846dff6104c62baf1b598edc29332 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14204096 14230143 |
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| DOI: | 10.1159/000527713 |