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Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions

التفاصيل البيبلوغرافية
العنوان: Carvedilol Ameliorates Experimental Atherosclerosis by Regulating Cholesterol Efflux and Exosome Functions
المؤلفون: Sy-Jou Chen, Pi-Fen Tsui, Yi-Ping Chuang, Dapi Meng-Lin Chiang, Liv Weichien Chen, Shu-Ting Liu, Feng-Yen Lin, Shih-Ming Huang, Shih-Hua Lin, Wan-Lin Wu, Min-Chien Tsai, Chin-Sheng Lin
المصدر: International Journal of Molecular Sciences, Vol 20, Iss 20, p 5202 (2019)
بيانات النشر: MDPI AG, 2019.
سنة النشر: 2019
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: carvedilol, atherosclerosis, cholesterol efflux, exosomes, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Carvedilol (Cav), a nonselective β-blocker with α1 adrenoceptor blocking effect, has been used as a standard therapy for coronary artery disease. This study investigated the effects of Cav on exosome expression and function, ATP-binding cassette transporter A1 (ABCA1) expression, and cholesterol efflux that are relevant to the process of atherosclerosis. Human monocytic (THP-1) cell line and human hepatic (Huh-7) cells were treated with Cav, and cholesterol efflux was measured. Exosomes from cell culture medium or mice serum were isolated using glycan-coated recognition beads. Low-density lipoprotein receptor knockout (ldlr−/−) mice were fed with high-fat diet and treated with Cav. Cav accentuated cholesterol efflux and enhanced the expressions of ABCA1 protein and mRNA in both THP-1 and Huh-7 cells. In addition, Cav increased expression and function of exosomal ABCA1 in THP-1 macrophage exosomes. The mechanisms were associated with inhibition of nuclear factor-κB (NF-κB) and protein kinase B (Akt). In hypercholesterolemic ldlr−/− mice, Cav enhanced serum exosomal ABCA1 expression and suppressed atherosclerosis by inhibiting lipid deposition and macrophage accumulation. Cav halts atherosclerosis by enhancing cholesterol efflux and increasing ABCA1 expression in macrophages and in exosomes, possibly through NF-κB and Akt signaling, which provides mechanistic insights regarding the beneficial effects of Cav on atherosclerotic cardiovascular disease.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/20/20/5202; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms20205202
URL الوصول: https://doaj.org/article/392db21fb57744ea945e65c000b17eb1
رقم الأكسشن: edsdoj.392db21fb57744ea945e65c000b17eb1
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms20205202