دورية أكاديمية
Regulation of Autophagy in Chick Skeletal Muscle: Effect of mTOR Inhibition
| العنوان: | Regulation of Autophagy in Chick Skeletal Muscle: Effect of mTOR Inhibition |
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| المؤلفون: | Kazuki Nakashima, Aiko Ishida |
| المصدر: | The Journal of Poultry Science, Vol 57, Iss 1, Pp 77-83 (2020) |
| بيانات النشر: | Japan Poultry Science Association, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Animal culture |
| مصطلحات موضوعية: | atrogin-1/mafbx, autophagy, chick myotubes, chick skeletal muscle, mechanistic target of rapamycin, torin1, Animal culture, SF1-1100 |
| الوصف: | Autophagy in the skeletal muscle increases under catabolic conditions resulting in muscle atrophy. This study investigated the effect of inhibition of mechanistic target of rapamycin (mTOR) on autophagy in chick skeletal muscle. We examined the effects of Torin1, an mTOR inhibitor, on autophagy. Chick myotubes were incubated with Torin1 (100 nM) for 3 h. It was observed that Torin1 inhibited the phosphorylation of AKT (Ser473), p70 ribosomal S6 kinase 1 (S6K1, Thr389), S6 ribosomal protein (Ser235/236), and eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1, Thr37/46), which are used for measurement of mTOR activity. Torin1 significantly (P< 0.01) increased the LC3-II/LC3-I ratio, an index for autophagosome formation, while it did not influence the expression of autophagy-related genes (LC3B, GABARAPL1, and ATG12). In addition, Torin1 increased atrogin-1/MAFbx (a muscle-specific ubiquitin ligase) mRNA expression. Fasting for 24 h inhibited the phosphorylation of AKT (Ser473), S6K1 (Ther389), S6 ribosomal protein (Ser235/236), and 4E-BP1 (Thr37/46) in chick skeletal muscle and significantly (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1346-7395 1349-0486 |
| Relation: | https://www.jstage.jst.go.jp/article/jpsa/57/1/57_0190008/_html/-char/en; https://doaj.org/toc/1346-7395; https://doaj.org/toc/1349-0486 |
| DOI: | 10.2141/jpsa.0190008 |
| URL الوصول: | https://doaj.org/article/39900450de4b4334a1fba96d195f0b62 |
| رقم الأكسشن: | edsdoj.39900450de4b4334a1fba96d195f0b62 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13467395 13490486 |
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| DOI: | 10.2141/jpsa.0190008 |