دورية أكاديمية
Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense
| العنوان: | Alu antisense RNA ameliorates methylglyoxal-induced human lens epithelial cell apoptosis by enhancing antioxidant defense |
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| المؤلفون: | Pei-Yuan Wu, Ning Ji, Chong-Guang Wu, Xiao-Die Wang, Xin Liu, Zhi-Xue Song, Murad Khan, Suleman Shah, Ying-Hua Du, Xiu-Fang Wang, Li-Fang Yan |
| المصدر: | International Journal of Ophthalmology, Vol 16, Iss 2, Pp 178-190 (2023) |
| بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Ophthalmology |
| مصطلحات موضوعية: | human alu antisense rna, human lens epithelial cells, methylglyoxal toxicity, antioxidant defense, apoptosis, Ophthalmology, RE1-994 |
| الوصف: | AIM: To determine whether an antisense RNA corresponding to the human Alu transposable element (Aluas RNA) can protect human lens epithelial cells (HLECs) from methylglyoxal-induced apoptosis. METHODS: Cell counting kit-8 (CCK-8) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to assess HLEC viability. HLEC viability/death was detected using a Calcein-AM/PI double staining kit; the annexin V-FITC method was used to detect HLEC apoptosis. The cytosolic reactive oxygen species (ROS) levels in HLECs were determined using a reactive species assay kit. The levels of malondialdehyde (MDA) and the antioxidant activities of total-superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) were assessed in HLECs using their respective kits. RT-qPCR and Western blotting were used to measure mRNA and protein expression levels of the genes. RESULTS: Aluas RNA rescued methylglyoxal-induced apoptosis in HLECs and ameliorated both the methylglyoxal-induced decrease in Bcl-2 mRNA and the methylglyoxal-induced increase in Bax mRNA. In addition, Aluas RNA inhibited the methylglyoxal-induced increase in Alu sense RNA expression. Aluas RNA inhibited the production of ROS induced by methylglyoxal, restored T-SOD and GSH-Px activity, and moderated the increase in MDA content after treatment with methylglyoxal. Aluas RNA significantly restored the methylglyoxal-induced down-regulation of Nrf2 gene and antioxidant defense genes, including glutathione peroxidase, heme oxygenase 1, γ-glutamylcysteine synthetase and quinone oxidoreductase 1. Aluas RNA ameliorated methylglyoxal-induced increases of the mRNA and protein expression of Keap1 that is the negative regulator of Nrf2. CONCLUSION: Aluas RNA reduces apoptosis induced by methylglyoxal by enhancing antioxidant defense. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2222-3959 2227-4898 |
| Relation: | http://ies.ijo.cn/en_publish/2023/2/20230203.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
| DOI: | 10.18240/ijo.2023.02.03 |
| URL الوصول: | https://doaj.org/article/39990438e917441a8bf5af6ec7ea0513 |
| رقم الأكسشن: | edsdoj.39990438e917441a8bf5af6ec7ea0513 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22223959 22274898 |
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| DOI: | 10.18240/ijo.2023.02.03 |