دورية أكاديمية
Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2.
العنوان: | Membrane progesterone receptor induces meiosis in Xenopus oocytes through endocytosis into signaling endosomes and interaction with APPL1 and Akt2. |
---|---|
المؤلفون: | Nancy Nader, Maya Dib, Rawad Hodeify, Raphael Courjaret, Asha Elmi, Ayat S Hammad, Raja Dey, Xin-Yun Huang, Khaled Machaca |
المصدر: | PLoS Biology, Vol 18, Iss 11, p e3000901 (2020) |
بيانات النشر: | Public Library of Science (PLoS), 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Biology (General) |
مصطلحات موضوعية: | Biology (General), QH301-705.5 |
الوصف: | The steroid hormone progesterone (P4) mediates many physiological processes through either nuclear receptors that modulate gene expression or membrane P4 receptors (mPRs) that mediate nongenomic signaling. mPR signaling remains poorly understood. Here we show that the topology of mPRβ is similar to adiponectin receptors and opposite to that of G-protein-coupled receptors (GPCRs). Using Xenopus oocyte meiosis as a well-established physiological readout of nongenomic P4 signaling, we demonstrate that mPRβ signaling requires the adaptor protein APPL1 and the kinase Akt2. We further show that P4 induces clathrin-dependent endocytosis of mPRβ into signaling endosome, where mPR interacts transiently with APPL1 and Akt2 to induce meiosis. Our findings outline the early steps involved in mPR signaling and expand the spectrum of mPR signaling through the multitude of pathways involving APPL1. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1544-9173 1545-7885 |
Relation: | https://doaj.org/toc/1544-9173; https://doaj.org/toc/1545-7885 |
DOI: | 10.1371/journal.pbio.3000901 |
URL الوصول: | https://doaj.org/article/da39de1e2d754001a84874e8d7594309 |
رقم الأكسشن: | edsdoj.39de1e2d754001a84874e8d7594309 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 15449173 15457885 |
---|---|
DOI: | 10.1371/journal.pbio.3000901 |