دورية أكاديمية
A genetic strategy for stochastic gene activation with regulated sparseness (STARS).
| العنوان: | A genetic strategy for stochastic gene activation with regulated sparseness (STARS). |
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| المؤلفون: | Sheng-Zhi Wang, Bao-Hua Liu, Huizhong W Tao, Kun Xia, Li I Zhang |
| المصدر: | PLoS ONE, Vol 4, Iss 1, p e4200 (2009) |
| بيانات النشر: | Public Library of Science (PLoS), 2009. |
| سنة النشر: | 2009 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | It remains a challenge to establish a straightforward genetic approach for controlling the probability of gene activation or knockout at a desired level. Here, we developed a method termed STARS: stochastic gene activation with genetically regulated sparseness. The stochastic expression was achieved by two cross-linked, mutually-exclusive Cre-mediated recombinations. The stochastic level was further controlled by regulating Cre/lox reaction kinetics through varying the intrachromosomal distance between the lox sites mediating one of the recombinations. In mammalian cell lines stably transfected with a single copy of different STARS transgenes, the activation/knockout of reporter genes was specifically controlled to occur in from 5% to 50% of the cell population. STARS can potentially provide a convenient way for genetic labeling as well as gene expression/knockout in a population of cells with a desired sparseness level. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC2615212?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0004200 |
| URL الوصول: | https://doaj.org/article/39f9e94178c04c2081e23378eefcf58f |
| رقم الأكسشن: | edsdoj.39f9e94178c04c2081e23378eefcf58f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0004200 |