دورية أكاديمية
Novel Zebrafish Mono-α2,8-sialyltransferase (ST8Sia VIII): An Evolutionary Perspective of α2,8-Sialylation
| العنوان: | Novel Zebrafish Mono-α2,8-sialyltransferase (ST8Sia VIII): An Evolutionary Perspective of α2,8-Sialylation |
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| المؤلفون: | Lan-Yi Chang, Elin Teppa, Maxence Noel, Pierre-André Gilormini, Mathieu Decloquement, Cédric Lion, Christophe Biot, Anne-Marie Mir, Virginie Cogez, Philippe Delannoy, Kay Hooi Khoo, Daniel Petit, Yann Guérardel, Anne Harduin-Lepers |
| المصدر: | International Journal of Molecular Sciences, Vol 20, Iss 3, p 622 (2019) |
| بيانات النشر: | MDPI AG, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | mono-α2,8-sialyltransferases, diSia motifs, evolution, ST8Sia, functional genomics, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The mammalian mono-α2,8-sialyltransferase ST8Sia VI has been shown to catalyze the transfer of a unique sialic acid residues onto core 1 O-glycans leading to the formation of di-sialylated O-glycosylproteins and to a lesser extent to diSia motifs onto glycolipids like GD1a. Previous studies also reported the identification of an orthologue of the ST8SIA6 gene in the zebrafish genome. Trying to get insights into the biosynthesis and function of the oligo-sialylated glycoproteins during zebrafish development, we cloned and studied this fish α2,8-sialyltransferase homologue. In situ hybridization experiments demonstrate that expression of this gene is always detectable during zebrafish development both in the central nervous system and in non-neuronal tissues. Intriguingly, using biochemical approaches and the newly developed in vitro MicroPlate Sialyltransferase Assay (MPSA), we found that the zebrafish recombinant enzyme does not synthetize diSia motifs on glycoproteins or glycolipids as the human homologue does. Using comparative genomics and molecular phylogeny approaches, we show in this work that the human ST8Sia VI orthologue has disappeared in the ray-finned fish and that the homologue described in fish correspond to a new subfamily of α2,8-sialyltransferase named ST8Sia VIII that was not maintained in Chondrichtyes and Sarcopterygii. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 20030622 |
| Relation: | https://www.mdpi.com/1422-0067/20/3/622; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms20030622 |
| URL الوصول: | https://doaj.org/article/3a094c769888453aa0b90128ecf2f795 |
| رقم الأكسشن: | edsdoj.3a094c769888453aa0b90128ecf2f795 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 20030622 |
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| DOI: | 10.3390/ijms20030622 |