دورية أكاديمية
Reducing self-shading effects in Botryococcus braunii cultures: effect of Mg2+ deficiency on optical and biochemical properties, photosynthesis and lipidomic profile
| العنوان: | Reducing self-shading effects in Botryococcus braunii cultures: effect of Mg2+ deficiency on optical and biochemical properties, photosynthesis and lipidomic profile |
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| المؤلفون: | Néstor David Giraldo, Sandra Marcela Correa, Andrés Arbeláez, Felix L. Figueroa, Rigoberto Ríos-Estepa, Lucía Atehortúa |
| المصدر: | Bioresources and Bioprocessing, Vol 8, Iss 1, Pp 1-14 (2021) |
| بيانات النشر: | SpringerOpen, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Technology LCC:Chemical technology LCC:Biotechnology |
| مصطلحات موضوعية: | Biofuels, Botryococcus braunii, Lipidomics, Mg2+ limitation, Photosynthesis, Self-shading effect, Technology, Chemical technology, TP1-1185, Biotechnology, TP248.13-248.65 |
| الوصف: | Abstract Microalgae biomass exploitation as a carbon–neutral energy source is currently limited by several factors, productivity being one of the most relevant. Due to the high absorption properties of light-harvesting antenna, photosynthetic cells tend to capture an excessive amount of energy that cannot be entirely channeled through the electron transfer chain that ends up dissipated as heat and fluorescence, reducing the overall light use efficiency. Aiming to minimize this hurdle, in this work we studied the effect of decreasing concentrations of Magnesium (Mg2+) on the chlorophyll a content, photosynthetic performance, biomass and lipid production of autotrophic cultures of Botryococcus braunii LB 572. We also performed, for the first time, a comparative lipidomic analysis to identify the influence of limited Mg2+ supply on the lipid profile of this algae. The results indicated that a level of 0.0037 g L−1 MgSO4 caused a significant decline on chlorophyll a content with a concomitant 2.3-fold reduction in the biomass absorption coefficient. In addition, the Mg2+ limitation caused a decrease in the total carbohydrate content and triggered lipid accumulation, achieving levels of up to 53% DCW, whereas the biomass productivity remained similar for all tested conditions. The lipidome analysis revealed that the lowest Mg2+ concentrations also caused a differential lipid profile distribution, with an enrichment of neutral lipids and an increase of structural lipids. In that sense, we showed that Mg2+ limitation represents an alternative optimization approach that not only enhances accumulation of neutral lipids in B. braunii cells but also may potentially lead to a better areal biomass productivity due to the reduction in the cellular light absorption properties of the cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2197-4365 |
| Relation: | https://doaj.org/toc/2197-4365 |
| DOI: | 10.1186/s40643-021-00389-z |
| URL الوصول: | https://doaj.org/article/e3a5bdc7656e47c59203768d6e170fcf |
| رقم الأكسشن: | edsdoj.3a5bdc7656e47c59203768d6e170fcf |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21974365 |
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| DOI: | 10.1186/s40643-021-00389-z |