دورية أكاديمية
The spatio-temporal expression analysis of parathyroid hormone like hormone gene provides a new insight for bone growth of the antler tip tissue in sika deer
| العنوان: | The spatio-temporal expression analysis of parathyroid hormone like hormone gene provides a new insight for bone growth of the antler tip tissue in sika deer |
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| المؤلفون: | Haihua Xing, Ruobing Han, Qianghui Wang, Zihui Sun, Heping Li |
| المصدر: | Animal Bioscience, Vol 37, Iss 8, Pp 1367-1376 (2024) |
| بيانات النشر: | Asian-Australasian Association of Animal Production Societies, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Zoology |
| مصطلحات موضوعية: | antlers, characterization, cloning, gene expression, sika deer, Zoology, QL1-991 |
| الوصف: | Objective Parathyroid hormone like hormone (PTHLH), as an essential factor for bone growth, is involved in a variety of physiological processes. The aim of this study was to explore the role of PTHLH gene in the growth of antlers. Methods The coding sequence (CDS) of PTHLH gene cDNA was obtained by cloning in sika deer (Cervus nippon), and the bioinformatics was analyzed. The quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the differences expression of PTHLH mRNA in different tissues of the antler tip at different growth periods (early period, EP; middle period, MP; late period, LP). Results The CDS of PTHLH gene was 534 bp in length and encoded 177 amino acids. Predictive analysis results revealed that the PTHLH protein was a hydrophilic protein without transmembrane structure, with its secondary structure consisting mainly of random coil. The PTHLH protein of sika deer had the identity of 98.31%, 96.82%, 96.05%, and 94.92% with Cervus canadensis, Bos mutus, Oryx dammah and Budorcas taxicolor, which were highly conserved among the artiodactyls. The qRT-PCR results showed that PTHLH mRNA had a unique spatio-temporal expression pattern in antlers. In the dermis, precartilage, and cartilage tissues, the expression of PTHLH mRNA was extremely significantly higher in MP than in EP, LP (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2765-0189 2765-0235 |
| Relation: | http://www.animbiosci.org/upload/pdf/ab-23-0421.pdf; https://doaj.org/toc/2765-0189; https://doaj.org/toc/2765-0235 |
| DOI: | 10.5713/ab.23.0421 |
| URL الوصول: | https://doaj.org/article/3a765e60c10e439d986782111fded62f |
| رقم الأكسشن: | edsdoj.3a765e60c10e439d986782111fded62f |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27650189 27650235 |
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| DOI: | 10.5713/ab.23.0421 |