دورية أكاديمية
VEGF and MMP-9 Gene Expression Caused by Treatment with Helicobacter Pylori Neutrophil-activating Recombinant Protein in a Breast Cancer Model
| العنوان: | VEGF and MMP-9 Gene Expression Caused by Treatment with Helicobacter Pylori Neutrophil-activating Recombinant Protein in a Breast Cancer Model |
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| المؤلفون: | N soleimani, B Farhangi, A Mohabati mobarez, F Etyabi, Z Hassan Saraf |
| المصدر: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 17, Iss 3, Pp 13-19 (2015) |
| بيانات النشر: | Babol University of Medical Sciences, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Medicine LCC:Medicine (General) |
| مصطلحات موضوعية: | Neutrophil-Activating Factor, Breast Cancer, Metastasis, Immunotherapy, Medicine, Medicine (General), R5-920 |
| الوصف: | BACKGROUND AND OBJECTIVE: Breast cancer is a major cause of death in women, worldwide. Cytotoxic drugs may lead to various adverse side-effects in patients. Helicobacter pylori neutrophil-activating protein (HP-NAP) is one of the most important proteins, produced by helicobacter pylori. The purpose of this study was to investigate the expression of VEGF and MMP-9 genes, caused by HP-NAP treatment in a breast cancer model. METHODS: In this experimental study, 18 female BALB/c mice, aged 6-8 weeks (19-23 g), were used. The mice were allocated to three groups: test group (treated with recombinant HP-NAP), positive control group (treated with an anti-cancer drug called trastuzumab), and negative control group (receiving phosphate-buffered saline). Expression and purification of HP-NAP were performed using nickel-resin affinity chromatography (Qiagen). The mice with breast tumors were treated with HP-NAP. MMP-9 and VEGF gene expression was measured using real-time polymerase chain reaction (PCR). FINDINGS: Protein expression was confirmed, using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The analysis showed the expression of a 20,000 dalton protein. Moreover, MMP-9 and VEGF gene expression decreased more significantly in the test group (due to treatment with HP-NAP), compared to the negative control group. The reduced expression in the test group was almost a quarter of the expression in the control group (p |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Persian |
| تدمد: | 1561-4107 2251-7170 |
| Relation: | http://jbums.org/browse.php?a_code=A-10-1564-1&slc_lang=en&sid=1; https://doaj.org/toc/1561-4107; https://doaj.org/toc/2251-7170 |
| URL الوصول: | https://doaj.org/article/3a9843b554014ac7ba32aeac00204483 |
| رقم الأكسشن: | edsdoj.3a9843b554014ac7ba32aeac00204483 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15614107 22517170 |
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