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Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing

التفاصيل البيبلوغرافية
العنوان: Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing
المؤلفون: Yan Zhang, Ping Zhou, Tohir A. Bozorov, Daoyuan Zhang
المصدر: Plant Methods, Vol 17, Iss 1, Pp 1-9 (2021)
بيانات النشر: BMC, 2021.
سنة النشر: 2021
المجموعة: LCC:Plant culture
LCC:Biology (General)
مصطلحات موضوعية: CRISPR/Cas9, Gene editing, Malus sieverii, PDS gene, Plant culture, SB1-1110, Biology (General), QH301-705.5
الوصف: Abstract Background Xinjiang wild apple is an important tree of the Tianshan Mountains, and in recent years, it has undergone destruction by many biotic and abiotic stress and human activities. It is necessary to use new technologies to research its genomic function and molecular improvement. The clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been successfully applied to genetic improvement in many crops, but its editing capability varies depending on the different combinations of the synthetic guide RNA (sgRNA) and Cas9 protein expression devices. Results In this study, we used 2 systems of vectors with paired sgRNAs targeting to MsPDS. As expected, we successfully induced the albino phenotype of calli and buds in both systems. Conclusions We conclude that CRISPR/Cas9 is a powerful system for editing the wild apple genome and expands the range of plants available for gene editing.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1746-4811
Relation: https://doaj.org/toc/1746-4811
DOI: 10.1186/s13007-021-00769-8
URL الوصول: https://doaj.org/article/d3ab52a6adec4214b5eef5cf940f76be
رقم الأكسشن: edsdoj.3ab52a6adec4214b5eef5cf940f76be
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17464811
DOI:10.1186/s13007-021-00769-8