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A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.

التفاصيل البيبلوغرافية
العنوان: A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.
المؤلفون: Sheng-Yi Wu, Yurong Wen, Nelson B C Serre, Cathrine Charlotte Heiede Laursen, Andrea Grostøl Dietz, Brian R Taylor, Mikhail Drobizhev, Rosana S Molina, Abhi Aggarwal, Vladimir Rancic, Michael Becker, Klaus Ballanyi, Kaspar Podgorski, Hajime Hirase, Maiken Nedergaard, Matyáš Fendrych, M Joanne Lemieux, Daniel F Eberl, Alan R Kay, Robert E Campbell, Yi Shen
المصدر: PLoS Biology, Vol 20, Iss 9, p e3001772 (2022)
بيانات النشر: Public Library of Science (PLoS), 2022.
سنة النشر: 2022
المجموعة: LCC:Biology (General)
مصطلحات موضوعية: Biology (General), QH301-705.5
الوصف: Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1544-9173
1545-7885
Relation: https://doaj.org/toc/1544-9173; https://doaj.org/toc/1545-7885
DOI: 10.1371/journal.pbio.3001772
URL الوصول: https://doaj.org/article/3acdec6eadb747dba6da2bd4ef33714b
رقم الأكسشن: edsdoj.3acdec6eadb747dba6da2bd4ef33714b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:15449173
15457885
DOI:10.1371/journal.pbio.3001772