دورية أكاديمية
أعرض في EDS

Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin

التفاصيل البيبلوغرافية
العنوان: Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin
المؤلفون: Jiankui Wang, Kai Cui, Zu Yang, Tun Li, Guoying Hua, Deping Han, Yanzhu Yao, Jianfei Chen, Xiaotian Deng, Xue Yang, Xuemei Deng
المصدر: International Journal of Molecular Sciences, Vol 20, Iss 3, p 620 (2019)
بيانات النشر: MDPI AG, 2019.
سنة النشر: 2019
المجموعة: LCC:Biology (General)
LCC:Chemistry
مصطلحات موضوعية: sheep, β-catenin, wool follicle, transgene, transcriptome, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: β-Catenin is an evolutionarily conserved molecule in the canonical Wnt signaling pathway, which controls decisive steps in embryogenesis and functions as a crucial effector in the development of hair follicles. However, the molecular mechanisms underlying wool production have not been fully elucidated. In this study, we investigated the effects of ovine β-catenin on wool follicles of transgenic sheep produced by pronuclear microinjection with a skin-specific promoter of human keratin14 (k14). Both polymerase chain reaction and Southern blot analysis showed that the sheep carried the ovine β-catenin gene and that the β-catenin gene could be stably inherited. To study the molecular responses to high expression of β-catenin, high-throughput RNA-seq technology was employed using three transgenic sheep and their wild-type siblings. These findings suggest that β-catenin normally plays an important role in wool follicle development by activating the downstream genes of the Wnt pathway and enhancing the expression of keratin protein genes and keratin-associated protein genes.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1422-0067
Relation: https://www.mdpi.com/1422-0067/20/3/620; https://doaj.org/toc/1422-0067
DOI: 10.3390/ijms20030620
URL الوصول: https://doaj.org/article/a3b1c6db5b53410f83cb5f4e4f3d342a
رقم الأكسشن: edsdoj.3b1c6db5b53410f83cb5f4e4f3d342a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:14220067
DOI:10.3390/ijms20030620