دورية أكاديمية
Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin
العنوان: | Transcriptome Analysis of Improved Wool Production in Skin-Specific Transgenic Sheep Overexpressing Ovine β-Catenin |
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المؤلفون: | Jiankui Wang, Kai Cui, Zu Yang, Tun Li, Guoying Hua, Deping Han, Yanzhu Yao, Jianfei Chen, Xiaotian Deng, Xue Yang, Xuemei Deng |
المصدر: | International Journal of Molecular Sciences, Vol 20, Iss 3, p 620 (2019) |
بيانات النشر: | MDPI AG, 2019. |
سنة النشر: | 2019 |
المجموعة: | LCC:Biology (General) LCC:Chemistry |
مصطلحات موضوعية: | sheep, β-catenin, wool follicle, transgene, transcriptome, Biology (General), QH301-705.5, Chemistry, QD1-999 |
الوصف: | β-Catenin is an evolutionarily conserved molecule in the canonical Wnt signaling pathway, which controls decisive steps in embryogenesis and functions as a crucial effector in the development of hair follicles. However, the molecular mechanisms underlying wool production have not been fully elucidated. In this study, we investigated the effects of ovine β-catenin on wool follicles of transgenic sheep produced by pronuclear microinjection with a skin-specific promoter of human keratin14 (k14). Both polymerase chain reaction and Southern blot analysis showed that the sheep carried the ovine β-catenin gene and that the β-catenin gene could be stably inherited. To study the molecular responses to high expression of β-catenin, high-throughput RNA-seq technology was employed using three transgenic sheep and their wild-type siblings. These findings suggest that β-catenin normally plays an important role in wool follicle development by activating the downstream genes of the Wnt pathway and enhancing the expression of keratin protein genes and keratin-associated protein genes. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1422-0067 |
Relation: | https://www.mdpi.com/1422-0067/20/3/620; https://doaj.org/toc/1422-0067 |
DOI: | 10.3390/ijms20030620 |
URL الوصول: | https://doaj.org/article/a3b1c6db5b53410f83cb5f4e4f3d342a |
رقم الأكسشن: | edsdoj.3b1c6db5b53410f83cb5f4e4f3d342a |
قاعدة البيانات: | Directory of Open Access Journals |
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