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Chronic mild stress-induced anhedonia in rats is coupled with the upregulation of inflammasome sensors: a possible involvement of NLRP1

التفاصيل البيبلوغرافية
العنوان: Chronic mild stress-induced anhedonia in rats is coupled with the upregulation of inflammasome sensors: a possible involvement of NLRP1
المؤلفون: Ceren Sahin Ozkartal, Feyza Aricioglu, Erdem Tuzun, Cem İsmail Kucukali
المصدر: Psychiatry and Clinical Psychopharmacology, Vol 28, Iss 3, Pp 236-244 (2018)
بيانات النشر: AVES, 2018.
سنة النشر: 2018
المجموعة: LCC:Neurosciences. Biological psychiatry. Neuropsychiatry
مصطلحات موضوعية: Stress, depression, NOD-like receptor, NLRP, inflammasome, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
الوصف: INTRODUCTION: NOD-like receptors containing pyrin domain (NLRP) are cytosolic receptors belong to innate immune system and function as sensing bodies for danger signals by forming inflammasome complex which in turn produces caspase-1-mediated interleukin (IL)-1β and IL-18 proinflammatory cytokines. Latest findings indicate that NLRP3 inflammasome mainly located in microglia cells in central nervous system (CNS) is linked to depression pathophysiology. However, another important CNS inflammasome, the neuronal NLRP1 inflammasome, has not been addressed in psychological stress or depression, yet. Therefore, the aim of the present study was to investigate the possible involvement NLRP1 inflammasome together with NLRP3 in chronic unpredictable mild stress (CUMS), a well-validated animal model of depression in rats. METHODS: Adult male Sprague–Dawley rats were divided into three groups: Control (treated with saline; non-stressed), CUMS (treated with saline), and CUMS + IMI (Imipramine; 10 mg/kg/day) (n = 6–8/group). In CUMS model, various stressors were applied for a total duration of six weeks. The treatments were daily administered via intraperitoneal (i.p.) route for the last three weeks of CUMS procedure. Anhedonia-like behaviors were assessed by sucrose preference test once in every two weeks throughout the experiment. At the end of the sixth week, rats were sacrificed and hippocampal brain tissues were collected for real-time PCR gene expression analysis of inflammasome components (NLRP1, NLRP3, ASC, and caspase-1) and inflammasome-dependent two proinflammatory cytokines (IL-1β and IL-18). RESULTS: CUMS-induced anhedonia in rats was coupled with upregulated mRNA levels of NLRP1, NLRP3, ASC, caspase-1, IL-1β, and IL-18 in hippocampus which were downregulated by chronic imipramine treatment. CONCLUSIONS: Our results suggest that the activation of not only NLRP3 but also NLRP1 inflammasome together may be involved in chronic stress-induced depression. Based on these results, further investigations are of great importance in order to understand the possible crosstalk between microglial (NLRP3) and neuronal (NLRP1) inflammasomes in depression and psychological stress.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2475-0573
2475-0581
24750573
Relation: https://doaj.org/toc/2475-0573; https://doaj.org/toc/2475-0581
DOI: 10.1080/24750573.2018.1426694
URL الوصول: https://doaj.org/article/3b9112581cb64ff3a16f3e388761a634
رقم الأكسشن: edsdoj.3b9112581cb64ff3a16f3e388761a634
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:24750573
24750581
DOI:10.1080/24750573.2018.1426694