دورية أكاديمية
Whole genome sequencing of simmental cattle for SNP and CNV discovery
| العنوان: | Whole genome sequencing of simmental cattle for SNP and CNV discovery |
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| المؤلفون: | Ting Sun, Shengwei Pei, Yangkai Liu, Quratulain Hanif, Haiyue Xu, Ningbo Chen, Chuzhao Lei, Xiangpeng Yue |
| المصدر: | BMC Genomics, Vol 24, Iss 1, Pp 1-11 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Genetics |
| مصطلحات موضوعية: | Simmental cattle, Selection, Sperm motility, SNP, CNV, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Abstract Backgroud The single nucleotide polymorphisms (SNPs) and copy number variations (CNVs) are two major genomic variants, which play crucial roles in evolutionary and phenotypic diversity. Results In this study, we performed a comprehensive analysis to explore the genetic variations (SNPs and CNVs) of high sperm motility (HSM) and poor sperm motility (PSM) Simmental bulls using the high-coverage (25×) short-read next generation sequencing and single-molecule long reads sequencing data. A total of ~ 15 million SNPs and 2,944 CNV regions (CNVRs) were detected in Simmental bulls, and a set of positive selected genes (PSGs) and CNVRs were found to be overlapped with quantitative trait loci (QTLs) involving immunity, muscle development, reproduction, etc. In addition, we detected two new variants in LEPR, which may be related to the artificial breeding to improve important economic traits. Moreover, a set of genes and pathways functionally related to male fertility were identified. Remarkably, a CNV on SPAG16 (chr2:101,427,468 − 101,429,883) was completely deleted in all poor sperm motility (PSM) bulls and half of the bulls in high sperm motility (HSM), which may play a crucial role in the bull-fertility. Conclusions In conclusion, this study provides a valuable genetic variation resource for the cattle breeding and selection programs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1471-2164 |
| Relation: | https://doaj.org/toc/1471-2164 |
| DOI: | 10.1186/s12864-023-09248-x |
| URL الوصول: | https://doaj.org/article/3c1eeb9481fa4069927d0c7e679b19bc |
| رقم الأكسشن: | edsdoj.3c1eeb9481fa4069927d0c7e679b19bc |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/s12864-023-09248-x |