دورية أكاديمية
Metagenomic next-generation sequencing for rapid detection of pulmonary infection in patients with acquired immunodeficiency syndrome
| العنوان: | Metagenomic next-generation sequencing for rapid detection of pulmonary infection in patients with acquired immunodeficiency syndrome |
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| المؤلفون: | Juan Zhong, Yanfen Liu, Na Luo, Qiu Wei, Qisi Su, Jun Zou, Xiaozhong Wu, Xianzhen Huang, Yuting Jiang, Lijuan Liang, Hongmian Li, Jianyan Lin |
| المصدر: | Annals of Clinical Microbiology and Antimicrobials, Vol 22, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Therapeutics. Pharmacology LCC:Infectious and parasitic diseases LCC:Microbiology |
| مصطلحات موضوعية: | Acquired immunodeficiency syndrome, Pulmonary infection, Metagenomic next-generation sequencing, Diagnosis, Therapeutics. Pharmacology, RM1-950, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502 |
| الوصف: | Abstract Background Acquired immunodeficiency syndrome (AIDS) is associated with a high rate of pulmonary infections (bacteria, fungi, and viruses). To overcome the low sensitivity and long turnaround time of traditional laboratory-based diagnostic strategies, we adopted metagenomic next-generation sequencing (mNGS) technology to identify and classify pathogens. Results This study enrolled 75 patients with AIDS and suspected pulmonary infections who were admitted to Nanning Fourth People’s Hospital. Specimens were collected for traditional microbiological testing and mNGS-based diagnosis. The diagnostic yields of the two methods were compared to evaluate the diagnostic value (detection rate and turn around time) of mNGS for infections with unknown causative agent. Accordingly, 22 cases (29.3%) had a positive culture and 70 (93.3%) had positive valve mNGS results (P value |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1476-0711 |
| Relation: | https://doaj.org/toc/1476-0711 |
| DOI: | 10.1186/s12941-023-00608-9 |
| URL الوصول: | https://doaj.org/article/3cf394a86b2141fdbce2406f0f40776f |
| رقم الأكسشن: | edsdoj.3cf394a86b2141fdbce2406f0f40776f |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 14760711 |
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| DOI: | 10.1186/s12941-023-00608-9 |