دورية أكاديمية
Genomic Characterization of IMP-Producing Pseudomonas aeruginosa in Bulgaria Reveals the Emergence of IMP-100, a Novel Plasmid-Mediated Variant Coexisting with a Chromosomal VIM-4
| العنوان: | Genomic Characterization of IMP-Producing Pseudomonas aeruginosa in Bulgaria Reveals the Emergence of IMP-100, a Novel Plasmid-Mediated Variant Coexisting with a Chromosomal VIM-4 |
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| المؤلفون: | Ivan Stoikov, Ivan N. Ivanov, Deyan Donchev, Deana Teneva, Elina Dobreva, Rumyana Hristova, Stefana Sabtcheva |
| المصدر: | Microorganisms, Vol 11, Iss 9, p 2270 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Pseudomonas, metallo-beta-lactamase, blaIMP, novel allele, plasmid, Biology (General), QH301-705.5 |
| الوصف: | Multidrug-resistant (MDR) Pseudomonas aeruginosa infections represent a major public health concern and require comprehensive understanding of their genetic makeup. This study investigated the first occurrence of imipenemase (IMP)-carrying P. aeruginosa strains from Bulgaria. Whole genome sequencing identified a novel plasmid-mediated IMP-100 allele located in a a novel In4886 integron embedded in a putative Tn7700 transposon. Two other closely related chromosomal IMP variants, IMP-13 and IMP-84, were also detected. The IMP-producers were resistant to last-line drugs including cefiderocol (CFDC) (two out of three) and susceptible to colistin. The IMP-13/84 cassettes were situated in a In320 integron inserted in a Tn5051-like transposon as previously reported. Lastly, the p4782-IMP plasmid rendered the PA01 transformant resistant to CFDC, suggesting a transferable CFDC resistance. A variety of virulence factors associated with adhesion, antiphagocytosis, iron uptake, and quorum sensing, as well as secretion systems, toxins, and proteases, were confirmed, suggesting significant pathogenic potential consistent with the observed strong biofilm formation. The emergence of IMP-producing MDR P. aeruginosa is alarming as it remains unsusceptible even to last-generation drugs like CFDC. Newly detected IMP-100 was even located in a CFDC-resistant XDR strain. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 11092270 2076-2607 |
| Relation: | https://www.mdpi.com/2076-2607/11/9/2270; https://doaj.org/toc/2076-2607 |
| DOI: | 10.3390/microorganisms11092270 |
| URL الوصول: | https://doaj.org/article/3d1d431d7b08408d82ca7cbd27a95fd7 |
| رقم الأكسشن: | edsdoj.3d1d431d7b08408d82ca7cbd27a95fd7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11092270 20762607 |
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| DOI: | 10.3390/microorganisms11092270 |