دورية أكاديمية
Long-Term Effects of Suramin on Renal Function in Streptozotocin-Induced Diabetes in Rats
| العنوان: | Long-Term Effects of Suramin on Renal Function in Streptozotocin-Induced Diabetes in Rats |
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| المؤلفون: | Gabriela Chyła-Danił, Kornelia Sałaga-Zaleska, Ewelina Kreft, Olaf Stumski, Aleksandra Krzesińska, Monika Sakowicz-Burkiewicz, Agnieszka Kuchta, Maciej Jankowski |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 19, p 14671 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | diabetes, nephropathy, chronic kidney disease, proteinuria, streptozotocin, suramin, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | In short-term diabetes (3 weeks), suramin, a drug used clinically, affects renal function and the expression of vascular endothelial growth factor A (VEGF-A), which may be involved in the pathogenesis of diabetic nephropathy, the main cause of end-stage renal disease. In the present study, we evaluated the long-term (11 weeks) effects of suramin (10 mg/kg, i.p., once-weekly) in diabetic rats. Concentrations of VEGF-A, albumin, soluble adhesive molecules (sICAM-1, sVCAM-1), nucleosomes, and thrombin–antithrombin complex (TAT) were measured by ELISA, total protein was measured using a biuret reagent. Glomerular expression of VEGF-A was evaluated by Western blot, mRNA for VEGF-A receptors in the renal cortex by RT-PCR. The vasoreactivity of the interlobar arteries to acetylcholine was assessed by wire myography. Long-term diabetes led to an increased concentration of VEGF-A, TAT, and urinary excretion of total protein and albumin, and a decrease in the concentration of sVCAM-1. We have shown that suramin in diabetes reduces total urinary protein excretion and restores the relaxing properties of acetylcholine relaxation properties to non-diabetic levels. Suramin had no effect on glomerular expression VEGF-A expression and specific receptors, and on sICAM-1 and nucleosomes concentrations in diabetic rats. In conclusion, the long-term effect of suramin on the kidneys in diabetes, expressed in the reduction of proteinuria and the restoration of endothelium-dependent relaxation of the renal arteries, can be considered as potentially contributing to the reduction/slowing down of the development of diabetic nephropathy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/19/14671; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms241914671 |
| URL الوصول: | https://doaj.org/article/3d77d31a2447435abdfc8b8521046d6a |
| رقم الأكسشن: | edsdoj.3d77d31a2447435abdfc8b8521046d6a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms241914671 |