دورية أكاديمية
Cell-derived vesicles from adipose-derived mesenchymal stem cells ameliorate irradiation-induced salivary gland cell damage
| العنوان: | Cell-derived vesicles from adipose-derived mesenchymal stem cells ameliorate irradiation-induced salivary gland cell damage |
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| المؤلفون: | Jeong Mi Kim, Mi Eun Choi, Eun Jeong Jeon, Jin-Mi Park, Sungryeal Kim, Jeong Eun Park, Seung Wook Oh, Jeong-Seok Choi |
| المصدر: | Regenerative Therapy, Vol 21, Iss , Pp 453-459 (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Cytology |
| مصطلحات موضوعية: | Salivary gland, Irradiation, Cell-derived vesicles (CDVs), Adipose-derived mesenchymal stem cells (ADMSCs), Medicine (General), R5-920, Cytology, QH573-671 |
| الوصف: | Introduction: Salivary gland (SG) damage is commonly caused by aging, irradiation, and some medications, and currently, no damage modifying agent is available. However, cell therapy based on mesenchymal stem cells (MSCs) has been proposed as a therapeutic modality for irradiated SGs. Therefore, we administered cell-derived vesicles (CDVs) of adipose-derived mesenchymal stem cells (ADMSCs) to irradiated SG cells to investigate their radioprotective effects in vitro. Methods: The artificial CDVs were obtained from ADMSC by tangential flow filtration (TFF) purification and ultracentrifugation. Cultured human SG epithelial cells were exposed to 2, 5 or 15 Gy of 4 MV X-rays produced by a linear accelerator. The effects of ADMSC-CDVs on SG epithelial cells damaged by irradiation were tested by proliferation activity, transepithelial electrical resistance (TEER), and amylase activity. Results: Exposure to penetrating radiation inhibited the proliferation of SG epithelial cells, but the radiation intensity required to reduce the proliferation of human submandibular gland epithelial cells (hSMGECs) was greater than required for other SG cells. ADMSC-CDVs restored the proliferative ability of SG epithelial cells reduced by irradiation, and the proliferation capacities of irradiated human parotid gland epithelial cells (hPGECs) and human sublingual gland epithelial cells (hSLGECs) were increased by administering ADMSC-CDVs to non-irradiated SG epithelial cells. Furthermore, amylase activity in irradiated hPGECs, hSMGECs, and hSLGECs was lower than in non-irradiated controls. However, amylase ability was restored in all by ADMSC-CDV treatment. Also, TEER was diminished by irradiation in hPGECs, hSMGECs, and hSLGECs and restored by ADMSC-CDV administration. Conclusion: Overall, our findings demonstrate that ADMSC-CDVs have potent radioprotective effects on irradiated SG cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3204 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352320422000931; https://doaj.org/toc/2352-3204 |
| DOI: | 10.1016/j.reth.2022.09.007 |
| URL الوصول: | https://doaj.org/article/3d9ec1535f704b6892761f11ce2052cd |
| رقم الأكسشن: | edsdoj.3d9ec1535f704b6892761f11ce2052cd |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523204 |
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| DOI: | 10.1016/j.reth.2022.09.007 |