دورية أكاديمية
The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria
| العنوان: | The Role of Mobile Genetic Elements in Virulence Factor Carriage from Symptomatic and Asymptomatic Cases of Escherichia coli Bacteriuria |
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| المؤلفون: | Grace Morales, Benjamin Abelson, Seth Reasoner, Jordan Miller, Ashlee M. Earl, Maria Hadjifrangiskou, Jonathan Schmitz |
| المصدر: | Microbiology Spectrum, Vol 11, Iss 3 (2023) |
| بيانات النشر: | American Society for Microbiology, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Microbiology |
| مصطلحات موضوعية: | AMR, antibiotic resistance, MGE, mobile genetic elements, UPEC, urinary tract infection, Microbiology, QR1-502 |
| الوصف: | ABSTRACT Uropathogenic Escherichia coli (UPEC) is extremely diverse genotypically and phenotypically. Individual strains can variably carry diverse virulence factors, making it challenging to define a molecular signature for this pathotype. For many bacterial pathogens, mobile genetic elements (MGEs) constitute a major mechanism of virulence factor acquisition. For urinary E. coli, the total distribution of MGEs and their role in the acquisition of virulence factors is not well defined, including in the context of symptomatic infection versus asymptomatic bacteriuria (ASB). In this work, we characterized 151 isolates of E. coli, derived from patients with either urinary tract infection (UTI) or ASB. For both sets of E. coli, we catalogued the presence of plasmids, prophage, and transposons. We analyzed MGE sequences for the presence of virulence factors and antimicrobial resistance genes. These MGEs were associated with only ~4% of total virulence associated genes, while plasmids contributed to ~15% of antimicrobial resistance genes under consideration. Our analyses suggests that, across strains of E. coli, MGEs are not a prominent driver of urinary tract pathogenesis and symptomatic infection. IMPORTANCE Escherichia coli is the most common etiological agent of urinary tract infections (UTIs), with UTI-associated strains designated “uropathogenic” E. coli or UPEC. Across urinary strains of E. coli, the global landscape of MGEs and its relationship to virulence factor carriage and clinical symptomatology require greater clarity. Here, we demonstrate that many of the putative virulence factors of UPEC are not associated with acquisition due to MGEs. The current work enhances our understanding of the strain-to-strain variability and pathogenic potential of urine-associated E. coli and points toward more subtle genomic differences distinguishing ASB from UTI isolates. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2165-0497 47769092 |
| Relation: | https://doaj.org/toc/2165-0497 |
| DOI: | 10.1128/spectrum.04710-22 |
| URL الوصول: | https://doaj.org/article/c3e5e1b2c87a477690927ed200ff709e |
| رقم الأكسشن: | edsdoj.3e5e1b2c87a477690927ed200ff709e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21650497 47769092 |
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| DOI: | 10.1128/spectrum.04710-22 |