دورية أكاديمية
Sivelestat improves acute lung injury by inhibiting PI3K/AKT/mTOR signaling pathway.
| العنوان: | Sivelestat improves acute lung injury by inhibiting PI3K/AKT/mTOR signaling pathway. |
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| المؤلفون: | Yaqing Zhou, Haiyan Wang, Aiming Liu, Zunguo Pu, Qiuxia Ji, Jianhua Xu, Yuehua Xu, Ying Wang |
| المصدر: | PLoS ONE, Vol 19, Iss 6, p e0302721 (2024) |
| بيانات النشر: | Public Library of Science (PLoS), 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | ObjectiveTo investigate the therapeutic effect and mechanism of sivelestat sodium on acute lung injury (AIL).MethodsA rat model for ALI/acute respiratory distress syndrome (ALI/ARDS) was established. Pathological examination of lung tissue was conducted to assess lung injury. Blood gas in the arteries was measured using a blood analyzer. Changes in PaO2, PaO2/FiO2, and lung wet/dry (W/D) weight ratio were carefully compared. ELISA assay was conducted to estimate cell adhesion and inflammation response. Finally, real-time reverse transcription polymerase chain reaction and western blotting assay was used to determine the activation of PI3K/AKT/mTOR pathway.ResultsARDS in vivo model was successfully constructed by LPS injection. Compared with the sham group, PaO2 and PaO2/FiO2 were significantly lower in the vehicle group, while the lung W/D ratio, the lung injury score, NE, VCAM-1, IL-8 andTNF-αwere significantly increased. After treatment with different doses of sivelestat sodium, we found PaO2, PaO2/FiO2 were prominently increased, while the lung W/D ratio, the lung injury score, NE, VCAM-1, IL-8, TNF-α levels were decreased in the dose-dependent manner. Meanwhile, compared with the vehicle group, the expression levels of Bax, PI3K, Akt and mTOR were significantly lower, and the expression of Bcl-2 was significantly higher after injection with sivelestat sodium.ConclusionSivelestat sodium has an interventional effect on ALI in sepsis by inhibiting the PI3K/AKT/mTOR signalling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0302721 |
| URL الوصول: | https://doaj.org/article/3e76561d998243e985083be1c0a50ab2 |
| رقم الأكسشن: | edsdoj.3e76561d998243e985083be1c0a50ab2 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0302721 |