دورية أكاديمية
miR-378a-5p inhibits the proliferation of colorectal cancer cells by downregulating CDK1
العنوان: | miR-378a-5p inhibits the proliferation of colorectal cancer cells by downregulating CDK1 |
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المؤلفون: | Kai Li, Jieling Zhang, Mingkang Zhang, Yaohua Wu, Xinyu Lu, Yiping Zhu |
المصدر: | World Journal of Surgical Oncology, Vol 19, Iss 1, Pp 1-13 (2021) |
بيانات النشر: | BMC, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Surgery LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
مصطلحات موضوعية: | miR-378a-5p, CDK1, CRC, Proliferation, Apoptosis, Surgery, RD1-811, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
الوصف: | Abstract Background MicroRNAs (miRNAs) play an important role in tumor occurrence. The role of miR-378a-5p and CDK1 in colorectal cancer (CRC) was investigated in this study. Methods Investigation of TCGA database and the detection of miR-378a-5p expression in colorectal cancer pathological tissues and colorectal cancer cell lines were undertaken by using qRT-PCR. We performed cell function experiments (CCK-8 assay, EdU assay, colony formation assay, wound healing assay, transwell assay, cell apoptosis assessment, and cell cycle assessment) and nude mouse tumor formation experiments to evaluate the effects of miR-378a-5p on proliferation, metastasis, and invasion to explore the role of miR-378a-5p in vivo and in vitro. Next, through TCGA database, immunohistochemical staining of pathological tissues, and cell function experiments, the role of the target gene CDK1 of miR-378a-5p was verified by database prediction, and dual luciferase reporter gene experiments in colorectal cancer cells were performed. Finally, whether upregulation of CDK1 restores the inhibitory effect of overexpression of miR-378a-5p on the proliferation of CRC cells was studied by overexpression of CDK1. Results Bioinformatic analysis showed significant downregulation of miR-378a-5p levels in colorectal cancer (CRC). Cell function experiments and tumor xenograft mouse models confirmed the low expression of miR-378a-5p within CRC tissues, which indicated the tumor suppressive role of miR-378a-5p in CRC. To better explore the regulation of miR-378a-5p in CRC, we predicted and validated cell cycle-dependent protein kinase 1 (CDK1) as the miR-378a-5p target gene and observed that miR-378a-5p suppressed CRC cell proliferation by targeting CDK1. Conclusion The results of this study help to elucidate the mechanism by which miR-378a-5p can be used as a tumor marker to inhibit the growth of colorectal cancer and CDK1, which is related to the prognosis of colorectal cancer patients. MiR-378a-5p inhibits CRC cell proliferation by suppressing CDK1 expression, which may become a possible therapeutic target for treatment of CRC. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1477-7819 |
Relation: | https://doaj.org/toc/1477-7819 |
DOI: | 10.1186/s12957-021-02166-w |
URL الوصول: | https://doaj.org/article/3eb48b71fe8a4787a4a2483b5d2eded2 |
رقم الأكسشن: | edsdoj.3eb48b71fe8a4787a4a2483b5d2eded2 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 14777819 |
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DOI: | 10.1186/s12957-021-02166-w |