دورية أكاديمية
Tetradesmus obliquus microalgae: solvent extraction of lipids under different process conditions
| العنوان: | Tetradesmus obliquus microalgae: solvent extraction of lipids under different process conditions |
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| المؤلفون: | Neuza Liliana Calvo Meroiço, Maurício de Oliveira Leite, César Augusto Sodré da Silva, Marcio Arêdes Martins, Monique Ellen Torres Resende, Eduardo Basílio de Oliveira, Jane Sélia dos Reis Coimbra |
| المصدر: | Scientia Agricola, Vol 81 (2024) |
| بيانات النشر: | Universidade de São Paulo, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Agriculture (General) |
| مصطلحات موضوعية: | ethanol, hexane, cell disruption, ball mill, lipoproteins, Agriculture (General), S1-972 |
| الوصف: | ABSTRACT Microalgal lipids exhibit physical-chemical characteristics similar to vegetable oils, representing alternative sources of long-chain fatty acids for food supplements. However, separating intracellular lipids from microalgae biomass is still challenging due to downstream processing technology limitations that depend on the particular microalgal species and its cultivation conditions. Thus, this work evaluated a multistep process for lipid extraction from Tetradesmus obliquus biomass instead of conventional single extraction methods to improve the lipid yield. The wet biomass was freeze-dried, the cells of the dried biomass were disrupted in a ball mill, and the milled biomass was suspended in water to obtain solid contents of 5 % and 10 % w v–1. Lipids were extracted from the aqueous suspension using the ethanol polar solvent and the hexane apolar solvent with ethanol:hexane ratios of 1:1, 1:2, and 3:5 v v–1. Tests were also carried out on the deproteinized biomass to evaluate the effect of proteins on emulsion formation since deproteinization can avoid emulsion formation. Thus, lipid downstream processing could be designed without an emulsion removal stage. The biomass was deproteinized before lipid extraction with NaOH at pH 10. The highest lipid content was verified for the 1:2 ethanol:hexane ratio, in which up to 42 % of the total lipids were extracted from the biomass. With the 3:5 ratio, the organic and aqueous phases emulsified due to the presence of lipoproteins, which hindered the lipid extraction process. Under the studied conditions, no increase in lipid extraction yield was observed with increases in the solid content or biomass deproteinization. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Spanish; Castilian Portuguese |
| تدمد: | 1678-992X 1678-992x |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162024000100903&lng=en&tlng=en; http://www.scielo.br/pdf/sa/v81/1678-992X-sa-81-e20220283.pdf; https://doaj.org/toc/1678-992X |
| DOI: | 10.1590/1678-992x-2022-0283 |
| URL الوصول: | https://doaj.org/article/3f11308e4cad4f6497e87d0838ce0987 |
| رقم الأكسشن: | edsdoj.3f11308e4cad4f6497e87d0838ce0987 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1678992X 1678992x |
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| DOI: | 10.1590/1678-992x-2022-0283 |